{"doi":"10.1126/sciimmunol.adq8843","title":"Proximity-dependent labeling identifies dendritic cells that drive the tumor-specific CD4 <sup>+</sup> T cell response","abstract":"Dendritic cells (DCs) are uniquely capable of transporting tumor antigens to tumor-draining lymph nodes (tdLNs) and interact with effector T cells in the tumor microenvironment (TME) itself, mediating both natural antitumor immunity and the response to checkpoint blockade immunotherapy. Using LIPSTIC (Labeling Immune Partnerships by SorTagging Intercellular Contacts)–based single-cell transcriptomics, we identified individual DCs capable of presenting antigen to CD4 + T cells in both the tdLN and TME. Our findings revealed that DCs with similar hyperactivated transcriptional phenotypes interact with helper T cells both in tumors and in the tdLN and that checkpoint blockade drugs enhance these interactions. These findings show that a relatively small fraction of DCs is responsible for most of the antigen presentation in the tdLN and TME to both CD4 + and CD8 + tumor–specific T cells and that classical checkpoint blockade enhances CD40-driven DC activation at both sites.","journal":"Science Immunology","year":2024,"id":422367,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":27,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9519,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":217900,"name":"Tiago B. R. Castro","orcid":"0000-0002-0359-0611","position":1,"is_corresponding":false},{"id":989454,"name":"Sandra Nakandakari-Higa","orcid":"0000-0001-8293-2992","position":2,"is_corresponding":false},{"id":239717,"name":"Ang Cui","orcid":"0000-0002-1087-8568","position":3,"is_corresponding":false},{"id":696723,"name":"Chia‐Hao Lin","orcid":"0000-0001-6510-8483","position":4,"is_corresponding":false},{"id":16005,"name":"Moshe Sade-Feldman","orcid":"0000-0002-0022-0287","position":5,"is_corresponding":false},{"id":854593,"name":"Brooke K. Phillips","orcid":"0000-0002-0109-0525","position":6,"is_corresponding":false},{"id":317092,"name":"Juhee Pae","orcid":"0000-0003-0676-0935","position":7,"is_corresponding":false},{"id":295162,"name":"Luka Mesin","orcid":"0000-0001-9852-6841","position":8,"is_corresponding":false},{"id":295163,"name":"Juliana Bortolatto","orcid":"0000-0001-8779-5464","position":9,"is_corresponding":false},{"id":7779,"name":"Lawrence D. Schweitzer","orcid":null,"position":10,"is_corresponding":false},{"id":989455,"name":"Giulia Pasqual","orcid":"0000-0002-1476-2669","position":11,"is_corresponding":false},{"id":236080,"name":"Li‐Fan Lu","orcid":"0000-0002-9727-0036","position":12,"is_corresponding":false},{"id":409,"name":"Nir Hacohen","orcid":"0000-0002-2349-2656","position":13,"is_corresponding":false},{"id":266895,"name":"Gabriel D. Victora","orcid":"0000-0001-8807-348X","position":14,"is_corresponding":false},{"id":266891,"name":"Aleksey Chudnovskiy","orcid":"0000-0002-5806-1725","position":0,"is_corresponding":true}],"reference_count":113,"raw_metadata":null,"created_at":"2026-07-19T01:57:46.393290Z","pmid":"39365874","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}