{"doi":"10.1126/sciimmunol.adi9066","title":"Zfp281 and Zfp148 control CD4 <sup>+</sup> T cell thymic development and T <sub>H</sub> 2 functions","abstract":"How CD4 + lineage gene expression is initiated in differentiating thymocytes remains poorly understood. Here, we show that the paralog transcription factors Zfp281 and Zfp148 control both this process and cytokine expression by T helper cell type 2 (T H 2) effector cells. Genetic, single-cell, and spatial transcriptomic analyses showed that these factors promote the intrathymic CD4 + T cell differentiation of class II major histocompatibility complex (MHC II)–restricted thymocytes, including expression of the CD4 + lineage–committing factor Thpok. In peripheral T cells, Zfp281 and Zfp148 promoted chromatin opening at and expression of T H 2 cytokine genes but not of the T H 2 lineage–determining transcription factor Gata3. We found that Zfp281 interacts with Gata3 and is recruited to Gata3 genomic binding sites at loci encoding Thpok and T H 2 cytokines. Thus, Zfp148 and Zfp281 collaborate with Gata3 to promote CD4 + T cell development and T H 2 cell responses.","journal":"Science Immunology","year":2023,"id":361399,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9557,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":234192,"name":"Xiaoliang Zhu","orcid":null,"position":1,"is_corresponding":false},{"id":299369,"name":"Yayi Gao","orcid":"0000-0003-4662-9552","position":2,"is_corresponding":false},{"id":712679,"name":"Jia Nie","orcid":"0000-0002-3865-0143","position":3,"is_corresponding":false},{"id":1113622,"name":"Jatinder Singh","orcid":"0000-0002-2605-7107","position":4,"is_corresponding":false},{"id":228410,"name":"Parimal Kumar","orcid":"0000-0003-1982-7983","position":5,"is_corresponding":false},{"id":368574,"name":"Kelly Z. Young","orcid":"0000-0002-6714-7563","position":6,"is_corresponding":false},{"id":623489,"name":"Shil Patel","orcid":"0000-0003-2672-8780","position":7,"is_corresponding":false},{"id":300505,"name":"Caiyi Li","orcid":null,"position":8,"is_corresponding":false},{"id":880672,"name":"Mariah Balmaceno-Criss","orcid":"0000-0002-5065-6062","position":9,"is_corresponding":false},{"id":712677,"name":"Melanie S. Vacchio","orcid":"0000-0001-5407-6439","position":10,"is_corresponding":false},{"id":212179,"name":"Michael M. Wang","orcid":"0000-0002-5670-2496","position":11,"is_corresponding":false},{"id":299370,"name":"Ferenc Livák","orcid":"0000-0003-2833-585X","position":12,"is_corresponding":false},{"id":279067,"name":"Juanita L. Merchant","orcid":"0000-0002-6559-8184","position":13,"is_corresponding":false},{"id":1111871,"name":"Lie Wang","orcid":"0000-0001-5094-012X","position":14,"is_corresponding":false},{"id":226280,"name":"Michael C. Kelly","orcid":"0000-0003-0654-2778","position":15,"is_corresponding":false},{"id":231485,"name":"Jinfang Zhu","orcid":"0000-0002-0117-4745","position":16,"is_corresponding":false},{"id":299371,"name":"Rémy Bosselut","orcid":"0000-0003-2033-1790","position":17,"is_corresponding":false},{"id":299366,"name":"Laura B. Chopp","orcid":"0000-0001-6494-4439","position":0,"is_corresponding":true}],"reference_count":85,"raw_metadata":null,"created_at":"2026-07-19T01:14:10.680991Z","pmid":"37948511","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}