{"doi":"10.1126/science.ady7227","title":"High-throughput single-cell omics using semipermeable capsules","abstract":"Biological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput single-cell omics methods and tools that efficiently probe the cellular phenotype and genotype. Here, we present a versatile technology based on semipermeable capsules (SPCs) tailored for a variety of high-throughput nucleic acid assays, including single-cell genome and mRNA sequencing, and fluorescence-activated cell sorting-based isolation of individual transcriptomes based on a nucleic acid marker of interest. Being biocompatible, the SPCs support single-cell cultivation and clonal expansion over long periods of time, thereby overcoming a fundamental limitation of droplet microfluidics platforms. Overall, the SPCs represent a customizable and broadly applicable tool for easy-to-use, scalable single-cell omics applications that are built on multistep biochemical reactions.","journal":"Science","year":2025,"id":513138,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9556,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1373484,"name":"Simonas Norvaisis","orcid":"0009-0000-0154-7970","position":1,"is_corresponding":false},{"id":1373485,"name":"Justina Žvirblytė","orcid":"0000-0001-8175-504X","position":2,"is_corresponding":false},{"id":1373486,"name":"Greta Leonavičienė","orcid":"0000-0002-3008-9603","position":3,"is_corresponding":false},{"id":1373487,"name":"Vincenta Mikulenaite","orcid":"0009-0003-1009-1751","position":4,"is_corresponding":false},{"id":1373488,"name":"Karolis Goda","orcid":"0009-0000-8276-6883","position":5,"is_corresponding":false},{"id":1373489,"name":"Vytautas Kašėta","orcid":"0000-0001-9702-3062","position":6,"is_corresponding":false},{"id":1373490,"name":"Karolis Šablauskas","orcid":"0000-0003-4166-0331","position":7,"is_corresponding":false},{"id":1373491,"name":"Laimonas Griškevičius","orcid":"0000-0002-3731-1537","position":8,"is_corresponding":false},{"id":571035,"name":"Simonas Juzėnas","orcid":"0000-0001-9293-0691","position":9,"is_corresponding":false},{"id":50789,"name":"Linas Mazutis","orcid":"0000-0002-5552-6427","position":10,"is_corresponding":false},{"id":1373483,"name":"Denis Baronas","orcid":"0000-0003-1878-0837","position":0,"is_corresponding":true}],"reference_count":87,"raw_metadata":null,"created_at":"2026-07-19T02:48:11.233009Z","pmid":"41411411","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}