{"doi":"10.1126/science.abp9262","title":"Single-cell analyses of axolotl telencephalon organization, neurogenesis, and regeneration","abstract":"<jats:p>Salamanders are tetrapod models to study brain organization and regeneration; however, the identity and evolutionary conservation of brain cell types are largely unknown. We delineated the cell populations in the axolotl telencephalon during homeostasis and regeneration using single-cell genomic profiling. We identified glutamatergic neurons with similarities to amniote neurons of hippocampus, dorsal and lateral cortex, and conserved γ-aminobutyric acid–releasing (GABAergic) neuron classes. We inferred transcriptional dynamics and gene regulatory relationships of postembryonic, region-specific neurogenesis and unraveled conserved differentiation signatures. After brain injury, ependymoglia activate an injury-specific state before reestablishing lost neuron populations and axonal connections. Together, our analyses yield insights into the organization, evolution, and regeneration of a tetrapod nervous system.</jats:p>","journal":"Science","year":2022,"id":608962,"datarank":2.5961474175485533,"base_score":4.700480365792417,"endowment":4.700480365792417,"self_citation_contribution":0.7050720548688626,"citation_network_contribution":1.8910753626796906,"self_endowment_contribution":0.7050720548688626,"citer_contribution":1.8910753626796906,"corpus_percentile":null,"corpus_rank":null,"citation_count":109,"citer_count":103,"citers_with_citation_signal":69,"citers_with_endowment":69,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":845567,"name":"Ashley Maynard","orcid":"0000-0002-3588-3080","position":1,"is_corresponding":false},{"id":31678,"name":"Tomás Gomes","orcid":"0000-0003-1333-0191","position":2,"is_corresponding":false},{"id":40551,"name":"Jonas Simon Fleck","orcid":"0000-0002-4686-7254","position":3,"is_corresponding":false},{"id":29631,"name":"J. Gray Camp","orcid":"0000-0003-3295-1225","position":4,"is_corresponding":false},{"id":598616,"name":"Elly M. Tanaka","orcid":"0000-0003-4240-2158","position":5,"is_corresponding":false},{"id":29636,"name":"Barbara Treutlein","orcid":"0000-0002-3299-5597","position":6,"is_corresponding":false},{"id":812463,"name":"Katharina Lust","orcid":"0000-0002-2580-5492","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Single-cell analyses of axolotl telencephalon organization, neurogenesis, and regeneration","abstract":"<jats:p>Salamanders are tetrapod models to study brain organization and regeneration; however, the identity and evolutionary conservation of brain cell types are largely unknown. We delineated the cell populations in the axolotl telencephalon during homeostasis and regeneration using single-cell genomic profiling. We identified glutamatergic neurons with similarities to amniote neurons of hippocampus, dorsal and lateral cortex, and conserved γ-aminobutyric acid–releasing (GABAergic) neuron classes. We inferred transcriptional dynamics and gene regulatory relationships of postembryonic, region-specific neurogenesis and unraveled conserved differentiation signatures. After brain injury, ependymoglia activate an injury-specific state before reestablishing lost neuron populations and axonal connections. Together, our analyses yield insights into the organization, evolution, and regeneration of a tetrapod nervous system.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36048956","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://www.science.org/doi/pdf/10.1126/science.abp9262","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Telencephalon","Neurons","Animals","Ambystoma mexicanum","Neurogenesis","Biological Evolution","Single-Cell Analysis","Brain Regeneration"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-31T01:15:28.424757Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}