{"doi":"10.1126/science.abm1184","title":"Csx28 is a membrane pore that enhances CRISPR-Cas13b–dependent antiphage defense","abstract":"Type VI CRISPR-Cas systems use RNA-guided ribonuclease (RNase) Cas13 to defend bacteria against viruses, and some of these systems encode putative membrane proteins that have unclear roles in Cas13-mediated defense. We show that Csx28, of type VI-B2 systems, is a transmembrane protein that assists to slow cellular metabolism upon viral infection, increasing antiviral defense. High-resolution cryo-electron microscopy reveals that Csx28 forms an octameric pore-like structure. These Csx28 pores localize to the inner membrane in vivo. Csx28's antiviral activity in vivo requires sequence-specific cleavage of viral messenger RNAs by Cas13b, which subsequently results in membrane depolarization, slowed metabolism, and inhibition of sustained viral infection. Our work suggests a mechanism by which Csx28 acts as a downstream, Cas13b-dependent effector protein that uses membrane perturbation as an antiviral defense strategy.","journal":"Science","year":2023,"id":323946,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9514,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":646122,"name":"Jung-Un Park","orcid":"0000-0002-0269-7142","position":1,"is_corresponding":false},{"id":865890,"name":"Bogdan Polevoda","orcid":"0000-0002-0743-4181","position":2,"is_corresponding":false},{"id":1039780,"name":"Julia K. Nicosia","orcid":null,"position":3,"is_corresponding":false},{"id":832410,"name":"Adrian M. Molina Vargas","orcid":"0000-0003-4090-1180","position":4,"is_corresponding":false},{"id":351499,"name":"Elizabeth H. Kellogg","orcid":"0000-0002-9664-3179","position":5,"is_corresponding":false},{"id":422293,"name":"Mitchell R. O’Connell","orcid":"0000-0002-8642-3973","position":6,"is_corresponding":false},{"id":1039319,"name":"Arica R. VanderWal","orcid":"0000-0001-6417-6046","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-19T01:08:06.496579Z","pmid":"37104586","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}