{"doi":"10.1126/science.abj4922","title":"Conformational selection guides β-arrestin recruitment at a biased G protein–coupled receptor","abstract":"G protein–coupled receptors (GPCRs) recruit β-arrestins to coordinate diverse cellular processes, but the structural dynamics driving this process are poorly understood. Atypical chemokine receptors (ACKRs) are intrinsically biased GPCRs that engage β-arrestins but not G proteins, making them a model system for investigating the structural basis of β-arrestin recruitment. Here, we performed nuclear magnetic resonance (NMR) experiments on 13 CH 3 -ε–methionine–labeled ACKR3, revealing that β-arrestin recruitment is associated with conformational exchange at key regions of the extracellular ligand-binding pocket and intracellular β-arrestin–coupling region. NMR studies of ACKR3 mutants defective in β-arrestin recruitment identified an allosteric hub in the receptor core that coordinates transitions among heterogeneously populated and selected conformational states. Our data suggest that conformational selection guides β-arrestin recruitment by tuning receptor dynamics at intracellular and extracellular regions.","journal":"Science","year":2022,"id":238892,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":52,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9605,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":827132,"name":"Shawn Jenjak","orcid":"0000-0002-2142-334X","position":1,"is_corresponding":false},{"id":863459,"name":"Andrija Sente","orcid":"0000-0001-5407-9138","position":2,"is_corresponding":false},{"id":227726,"name":"Lauren J. Laskowski","orcid":"0000-0003-3979-7507","position":3,"is_corresponding":false},{"id":442133,"name":"Martyna Szpakowska","orcid":"0000-0002-5659-8377","position":4,"is_corresponding":false},{"id":863460,"name":"Maggie M. Calkins","orcid":"0000-0001-5639-0739","position":5,"is_corresponding":false},{"id":229727,"name":"Emilie I. Anderson","orcid":null,"position":6,"is_corresponding":false},{"id":864094,"name":"Lisa McNally","orcid":null,"position":7,"is_corresponding":false},{"id":863461,"name":"Raimond Heukers","orcid":"0000-0003-4498-7751","position":8,"is_corresponding":false},{"id":863462,"name":"Vladimir Bobkov","orcid":"0000-0002-5414-8188","position":9,"is_corresponding":false},{"id":275184,"name":"Francis C. Peterson","orcid":"0000-0001-9890-4224","position":10,"is_corresponding":false},{"id":439420,"name":"Monica A. Thomas","orcid":"0000-0003-2513-3371","position":11,"is_corresponding":false},{"id":442134,"name":"Andy Chevigné","orcid":"0000-0003-4768-6743","position":12,"is_corresponding":false},{"id":863463,"name":"Martine J. Smit","orcid":"0000-0003-2713-0238","position":13,"is_corresponding":false},{"id":225472,"name":"John D. McCorvy","orcid":"0000-0001-7555-9413","position":14,"is_corresponding":false},{"id":91395,"name":"M. Madan Babu","orcid":"0000-0003-0556-6196","position":15,"is_corresponding":false},{"id":275185,"name":"Brian F. Volkman","orcid":"0000-0002-6681-5179","position":16,"is_corresponding":false},{"id":275182,"name":"Andrew B. Kleist","orcid":"0000-0002-4954-010X","position":0,"is_corresponding":true}],"reference_count":91,"raw_metadata":null,"created_at":"2026-07-19T00:22:31.741535Z","pmid":"35857540","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}