{"doi":"10.1126/science.abi8976","title":"Structural basis for target site selection in RNA-guided DNA transposition systems","abstract":"Target site selection in CAST systems Exciting genomic engineering possibilities exist for natural integration systems called transposons, which have co-opted CRISPR/Cas systems. An unexplained feature of these systems involves how they direct insertions in a single orientation at a precise distance from the programmed target sequence. Park et al . show that orientation information is communicated to the transposase, TnsB, using the unidirectional growth of a helical filament made up of an AAA+ protein, TnsC. ATP hydrolysis trims the filament to a minimal unit that is marked by TniQ and defined by the Cas12k protein to provide spacing information. This finding may help future engineering of these systems for therapeutic applications. —DJ","journal":"Science","year":2021,"id":152070,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":78,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9405,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":646123,"name":"Amy Wei-Lun Tsai","orcid":"0000-0001-8856-617X","position":1,"is_corresponding":false},{"id":646124,"name":"Eshan Mehrotra","orcid":"0000-0003-3153-9570","position":2,"is_corresponding":false},{"id":279043,"name":"Michael T. Petassi","orcid":"0000-0002-4934-8636","position":3,"is_corresponding":false},{"id":279044,"name":"Shan-Chi Hsieh","orcid":"0000-0002-9480-9024","position":4,"is_corresponding":false},{"id":646125,"name":"Ailong Ke","orcid":"0000-0001-5957-7822","position":5,"is_corresponding":false},{"id":279045,"name":"Joseph E. Peters","orcid":"0000-0001-8309-3297","position":6,"is_corresponding":false},{"id":351499,"name":"Elizabeth H. Kellogg","orcid":"0000-0002-9664-3179","position":7,"is_corresponding":false},{"id":646122,"name":"Jung-Un Park","orcid":"0000-0002-0269-7142","position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-18T23:43:22.562252Z","pmid":"34385391","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}