{"doi":"10.1126/science.abi8358","title":"Mapping the catalytic conformations of an assembly-line polyketide synthase module","abstract":"Assembly-line polyketide synthases, such as the 6-deoxyerythronolide B synthase (DEBS), are large enzyme factories prized for their ability to produce specific and complex polyketide products. By channeling protein-tethered substrates across multiple active sites in a defined linear sequence, these enzymes facilitate programmed small-molecule syntheses that could theoretically be harnessed to access countless polyketide product structures. Using cryogenic electron microscopy to study DEBS module 1, we present a structural model describing this substrate-channeling phenomenon. Our 3.2- to 4.3-angstrom-resolution structures of the intact module reveal key domain-domain interfaces and highlight an unexpected module asymmetry. We also present the structure of a product-bound module that shines light on a recently described “turnstile” mechanism for transient gating of active sites along the assembly line.","journal":"Science","year":2021,"id":151018,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":87,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9502,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":228228,"name":"Kaiming Zhang","orcid":"0000-0003-0414-4776","position":1,"is_corresponding":false},{"id":430076,"name":"Xiuyuan Li","orcid":"0000-0002-3970-8183","position":2,"is_corresponding":false},{"id":228230,"name":"Shanshan Li","orcid":"0000-0002-7041-5960","position":3,"is_corresponding":false},{"id":228227,"name":"Grigore Pintilie","orcid":"0000-0002-0848-5335","position":4,"is_corresponding":false},{"id":284273,"name":"Soung‐Hun Roh","orcid":"0000-0003-3851-7200","position":5,"is_corresponding":false},{"id":288757,"name":"Charles S. Craik","orcid":"0000-0001-7704-9185","position":6,"is_corresponding":false},{"id":91649,"name":"Wah Chiu","orcid":"0000-0002-8910-3078","position":7,"is_corresponding":false},{"id":430078,"name":"Chaitan Khosla","orcid":"0000-0001-6529-495X","position":8,"is_corresponding":false},{"id":417667,"name":"D.P. Cogan","orcid":"0000-0003-4563-2397","position":0,"is_corresponding":true}],"reference_count":70,"raw_metadata":null,"created_at":"2026-07-18T23:43:11.293086Z","pmid":"34735239","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}