{"doi":"10.1126/science.abg3875","title":"Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon","abstract":"The elongation of eukaryotic selenoproteins relies on a poorly understood process of interpreting in-frame UGA stop codons as selenocysteine (Sec). We used cryo-electron microscopy to visualize Sec UGA recoding in mammals. A complex between the noncoding Sec-insertion sequence (SECIS), SECIS-binding protein 2 (SBP2), and 40 S ribosomal subunit enables Sec-specific elongation factor eEFSec to deliver Sec. eEFSec and SBP2 do not interact directly but rather deploy their carboxyl-terminal domains to engage with the opposite ends of the SECIS. By using its Lys-rich and carboxyl-terminal segments, the ribosomal protein eS31 simultaneously interacts with Sec-specific transfer RNA (tRNA Sec ) and SBP2, which further stabilizes the assembly. eEFSec is indiscriminate toward l -serine and facilitates its misincorporation at Sec UGA codons. Our results support a fundamentally distinct mechanism of Sec UGA recoding in eukaryotes from that in bacteria.","journal":"Science","year":2022,"id":236733,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":66,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9513,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":857732,"name":"Benjamin Y. Killam","orcid":"0000-0002-7774-9648","position":1,"is_corresponding":false},{"id":357504,"name":"Milica Grozdanović","orcid":null,"position":2,"is_corresponding":false},{"id":857733,"name":"Malgorzata Dobosz-Bartoszek","orcid":"0000-0001-7773-596X","position":3,"is_corresponding":false},{"id":857734,"name":"J. Loerke","orcid":"0000-0002-3424-8536","position":4,"is_corresponding":false},{"id":270922,"name":"Jörg Bürger","orcid":"0000-0002-4326-5047","position":5,"is_corresponding":false},{"id":270923,"name":"Thorsten Mielke","orcid":"0000-0001-9275-3146","position":6,"is_corresponding":false},{"id":179568,"name":"Paul R. Copeland","orcid":"0000-0003-3343-0587","position":7,"is_corresponding":false},{"id":857735,"name":"Miljan Simonović","orcid":"0000-0002-1576-2222","position":8,"is_corresponding":false},{"id":636301,"name":"C.M.T. Spahn","orcid":"0000-0002-2939-9487","position":9,"is_corresponding":false},{"id":270920,"name":"Tarek Hilal","orcid":"0000-0002-7833-8058","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-19T00:22:03.686498Z","pmid":"35709277","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}