{"doi":"10.1126/science.abd9909","title":"De novo design of picomolar SARS-CoV-2 miniprotein inhibitors","abstract":"<jats:title>Miniproteins against SARS-CoV-2</jats:title>\n                  <jats:p>\n                    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is decorated with spikes, and viral entry into cells is initiated when these spikes bind to the host angiotensin-converting enzyme 2 (ACE2) receptor. Many monoclonal antibody therapies in development target the spike proteins. Cao\n                    <jats:italic>et al.</jats:italic>\n                    designed small, stable proteins that bind tightly to the spike and block it from binding to ACE2. The best designs bind with very high affinity and prevent SARS-CoV-2 infection of mammalian Vero E6 cells. Cryo–electron microscopy shows that the structures of the two most potent inhibitors are nearly identical to the computational models. Unlike antibodies, the miniproteins do not require expression in mammalian cells, and their small size and high stability may allow formulation for direct delivery to the nasal or respiratory system.\n                  </jats:p>\n                  <jats:p>\n                    <jats:italic>Science</jats:italic>\n                    , this issue p.\n                    <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" issue=\"6515\" page=\"426\" related-article-type=\"in-this-issue\" vol=\"370\" xlink:href=\"10.1126/science.abd9909\">426</jats:related-article>\n                  </jats:p>","journal":"Science","year":2020,"id":47287,"datarank":0.0,"base_score":6.588926477533519,"endowment":6.588926477533519,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":726,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":12,"is_dataset":false,"is_dataset_confidence":0.9497,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":218558,"name":"Inna Goreshnik","orcid":"0000-0002-9254-9249","position":1,"is_corresponding":false},{"id":218559,"name":"Brian Coventry","orcid":"0000-0002-6910-6255","position":2,"is_corresponding":false},{"id":106498,"name":"James Brett Case","orcid":"0000-0001-7331-5511","position":3,"is_corresponding":false},{"id":218560,"name":"L. M. Miller","orcid":"0000-0001-5935-4549","position":4,"is_corresponding":false},{"id":218561,"name":"Lisa Kozodoy","orcid":null,"position":5,"is_corresponding":false},{"id":106500,"name":"Rita E. Chen","orcid":"0000-0002-5100-7750","position":6,"is_corresponding":false},{"id":108913,"name":"Lauren Carter","orcid":"0000-0002-9837-9068","position":7,"is_corresponding":false},{"id":97046,"name":"Alexandra C. Walls","orcid":"0000-0002-9636-8330","position":8,"is_corresponding":false},{"id":97052,"name":"Young‐Jun Park","orcid":"0000-0003-2901-6949","position":9,"is_corresponding":false},{"id":218563,"name":"Eva‐Maria Strauch","orcid":"0000-0001-7382-747X","position":10,"is_corresponding":false},{"id":218564,"name":"Lance Stewart","orcid":"0000-0003-4264-5125","position":11,"is_corresponding":false},{"id":106529,"name":"Michael Diamond","orcid":"0000-0002-8791-3165","position":12,"is_corresponding":false},{"id":97051,"name":"David Veesler","orcid":"0000-0002-6019-8675","position":13,"is_corresponding":false},{"id":105706,"name":"David Baker","orcid":"0000-0001-7896-6217","position":14,"is_corresponding":false},{"id":218557,"name":"Longxing Cao","orcid":"0000-0003-4002-3648","position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":null,"created_at":"2026-07-18T14:18:31.320809Z","pmid":"32907861","pmcid":null,"fwci":11.4008,"citation_percentile":0.98657196,"influential_citations":20,"oa_status":"hybrid","license":"cc-by","views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.16689854.v1","title":"Additional file 1 of Enumeration and comprehensive in-silico modeling of three-helix bundle structures composed of typical αα-hairpins","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.16689854","title":"Additional file 1 of Enumeration and comprehensive in-silico 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