{"doi":"10.1126/science.abb2507","title":"Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation","abstract":"The outbreak of a novel coronavirus (2019-nCoV) represents a pandemic threat that has been declared a public health emergency of international concern. The CoV spike (S) glycoprotein is a key target for vaccines, therapeutic antibodies, and diagnostics. To facilitate medical countermeasure development, we determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation. The predominant state of the trimer has one of the three receptor-binding domains (RBDs) rotated up in a receptor-accessible conformation. We also provide biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S. Additionally, we tested several published SARS-CoV RBD-specific monoclonal antibodies and found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs. The structure of 2019-nCoV S should enable the rapid development and evaluation of medical countermeasures to address the ongoing public health crisis.","journal":"Science","year":2020,"id":10536,"datarank":23.890080802609607,"base_score":9.183688340978756,"endowment":9.183688340978756,"self_citation_contribution":1.3775532511468136,"citation_network_contribution":22.512527551462792,"self_endowment_contribution":1.3775532511468136,"citer_contribution":22.512527551462792,"corpus_percentile":96.6,"corpus_rank":1171,"citation_count":9736,"citer_count":190,"citers_with_citation_signal":190,"citers_with_endowment":190,"datacite_reuse_total":0,"is_dataset":false,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-03-13","authors":[{"id":87306,"name":"Nianshuang Wang","orcid":"0000-0002-7569-011X","position":1,"is_corresponding":false},{"id":87307,"name":"Kizzmekia S. Corbett","orcid":"0000-0002-2641-4688","position":2,"is_corresponding":false},{"id":87308,"name":"Jory A. Goldsmith","orcid":"0000-0003-2917-0620","position":3,"is_corresponding":false},{"id":87309,"name":"Ching-Lin Hsieh","orcid":null,"position":4,"is_corresponding":false},{"id":87310,"name":"Olubukola M. Abiona","orcid":"0000-0002-2745-1691","position":5,"is_corresponding":false},{"id":87311,"name":"Barney S. Graham","orcid":"0000-0001-8112-0853","position":6,"is_corresponding":false},{"id":87312,"name":"Jason S. McLellan","orcid":"0000-0003-3991-542X","position":7,"is_corresponding":false},{"id":87313,"name":"Ching‐Lin Hsieh","orcid":"0000-0002-3665-5717","position":8,"is_corresponding":false},{"id":87305,"name":"Daniel Wrapp","orcid":"0000-0002-0538-9647","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}