{"doi":"10.1126/science.aav7003","title":"Structures and operating principles of the replisome","abstract":"<jats:title>Structures of the simplest replisome</jats:title>\n                  <jats:p>\n                    The DNA replisome performs concerted parental-strand separation and DNA synthesis on both strands. Gao\n                    <jats:italic>et al.</jats:italic>\n                    report the cryo–electron microscopy structures of the minimum set of bacteriophage T7 proteins that can carry out leading- and lagging-strand synthesis at the replication fork (see the Perspective by Li and O'Donnell). Three key enzymes involved in DNA replication—DNA polymerase, helicase, and primase—were visualized in complex with substrate DNA, demonstrating their highly dynamic organizations on both strands. Comparison of prokaryotic and eukaryotic replisomes reveals evolutionarily conserved operating principles and provides a structural basis for understanding coordination among DNA replication, recombination, and repair.\n                  </jats:p>\n                  <jats:p>\n                    <jats:italic>Science</jats:italic>\n                    , this issue p.\n                    <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" related-article-type=\"in-this-issue\" xlink:href=\"10.1126/science.aav7003\">eaav7003</jats:related-article>\n                    ; see also p.\n                    <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" issue=\"6429\" page=\"814\" related-article-type=\"in-this-issue\" vol=\"363\" xlink:href=\"10.1126/science.aaw6265\">814</jats:related-article>\n                  </jats:p>","journal":"Science","year":2019,"id":598211,"datarank":0.7667981682534817,"base_score":5.111987788356544,"endowment":5.111987788356544,"self_citation_contribution":0.7667981682534817,"citation_network_contribution":0.0,"self_endowment_contribution":0.7667981682534817,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":165,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":305638,"name":"Yanxiang Cui","orcid":"0000-0002-8909-6576","position":1,"is_corresponding":false},{"id":262653,"name":"Tara Fox","orcid":"0000-0002-9324-1756","position":2,"is_corresponding":false},{"id":1532782,"name":"Shiqiang Lin","orcid":"0000-0001-7181-4261","position":3,"is_corresponding":false},{"id":263688,"name":"Huaibin Wang","orcid":"0000-0002-7466-0090","position":4,"is_corresponding":false},{"id":228429,"name":"Natalia de Val","orcid":"0000-0002-1506-2023","position":5,"is_corresponding":false},{"id":266761,"name":"Z. Hong Zhou","orcid":"0000-0002-8373-4717","position":6,"is_corresponding":false},{"id":263690,"name":"Wei Yang","orcid":"0000-0002-3591-2195","position":7,"is_corresponding":false},{"id":678959,"name":"Yang Gao","orcid":"0000-0002-4037-0431","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Structures and operating principles of the replisome","abstract":"<jats:title>Structures of the simplest replisome</jats:title>\n                  <jats:p>\n                    The DNA replisome performs concerted parental-strand separation and DNA synthesis on both strands. Gao\n                    <jats:italic>et al.</jats:italic>\n                    report the cryo–electron microscopy structures of the minimum set of bacteriophage T7 proteins that can carry out leading- and lagging-strand synthesis at the replication fork (see the Perspective by Li and O'Donnell). Three key enzymes involved in DNA replication—DNA polymerase, helicase, and primase—were visualized in complex with substrate DNA, demonstrating their highly dynamic organizations on both strands. Comparison of prokaryotic and eukaryotic replisomes reveals evolutionarily conserved operating principles and provides a structural basis for understanding coordination among DNA replication, recombination, and repair.\n                  </jats:p>\n                  <jats:p>\n                    <jats:italic>Science</jats:italic>\n                    , this issue p.\n                    <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" related-article-type=\"in-this-issue\" xlink:href=\"10.1126/science.aav7003\">eaav7003</jats:related-article>\n                    ; see also p.\n                    <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" issue=\"6429\" page=\"814\" related-article-type=\"in-this-issue\" vol=\"363\" xlink:href=\"10.1126/science.aaw6265\">814</jats:related-article>\n                  </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30679383","pmcid":"PMC6681829","openalex_id":null,"authors":[],"funders":[{"funder_name":"National Science Foundation","grant_id":"DBI-1338135","title":null},{"funder_name":"National Institutes of Health","grant_id":"Intramural DK036146","title":null},{"funder_name":"National Institutes of Health","grant_id":"GM071940","title":null},{"funder_name":"National Institutes of Health","grant_id":"1S10OD018111","title":null},{"funder_name":"National Institutes of Health","grant_id":"1S10RR23057","title":null},{"funder_name":"National Institutes of Health","grant_id":"intramural NCI funding","title":null},{"funder_name":"NIH HHS","grant_id":"S10 OD018111","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"ZIA DK036146","title":null},{"funder_name":"Intramural NIH HHS","grant_id":"ZIA DK075037","title":null},{"funder_name":"NCRR NIH HHS","grant_id":"S10 RR023057","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"U24 GM116792","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM071940","title":null},{"funder_name":"National Institutes of Health","grant_id":"1ZIADK036146-09","title":"Structural and mechanistic studies of translesion DNA synthesis"},{"funder_name":"National Institutes of Health","grant_id":"1S10OD018111-01","title":"Direct Detection Device for atomic resolution cryoEM of macromolecular complexes"},{"funder_name":"National Institutes of Health","grant_id":"1S10RR023057-01","title":"High-End CryoEM Instrument for the UCLA Electron Imaging Center for Nanomachines"},{"funder_name":"National Science Foundation","grant_id":"1338135","title":"MRI: Acquisition of a direct electron detector for the UCLA Titan electron microscope"}],"total_grants":16,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":null,"oa_locations":[{"url":"https://escholarship.org/content/qt7f4377zq/qt7f4377zq.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1126/science.aav7003","host_type":"publisher"},{"url":"https://www.science.org/doi/pdf/10.1126/science.aav7003","host_type":"publisher"},{"url":"https://doi.org/10.1126/science.aav7003","host_type":""},{"url":"https://europepmc.org/articles/pmc6681829?pdf=render","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/30679383","host_type":""},{"url":"https://dx.doi.org/10.1126/science.aav7003","host_type":""},{"url":"https://escholarship.org/uc/item/7f4377zq","host_type":""},{"url":"https://doi.org/https://doi.org/10.1126/science.aav7003","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Bacteriophage T7","DNA Helicases","DNA-Directed DNA Polymerase","DNA Primase","Viral Proteins","Cryoelectron Microscopy","Virus Replication","Protein Domains"],"keywords":["General Science & Technology","Cryoelectron Microscopy","DNA Helicases","500","DNA Primase","DNA-Directed DNA Polymerase","Biological Sciences","540","Virus Replication","Viral Proteins","Protein Domains","Bacteriophage T7","Physical Sciences","Genetics","Biochemistry and Cell Biology"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T15:17:05.903213Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}