{"doi":"10.1126/science.3107122","title":"Insulin Rapidly Increases Diacylglycerol by Activating De Novo Phosphatidic Acid Synthesis","abstract":"<jats:p>\n            The mechanisms whereby insulin increases diacylglycerol in BC3H-1 myocytes were examined. When [\n            <jats:sup>3</jats:sup>\n            H]arachidonate labeling of phospholipids was used as an indicator of phospholipase C activation, transient increases in [\n            <jats:sup>3</jats:sup>\n            H]diacylglycerol were observed between 0.5 and 10 minutes after the onset of insulin treatment. With [\n            <jats:sup>3</jats:sup>\n            H]glycerol labeling as an indicator of de novo phospholipid synthesis, [\n            <jats:sup>3</jats:sup>\n            H]diacylglycerol was increased maximally at 1 minute and remained elevated for 20 minutes. [\n            <jats:sup>3</jats:sup>\n            H]Glycerol-labeled diacylglycerol was largely derived directly from phosphatidic acid. Insulin increased de novo phosphatidic acid synthesis within 5 to 10 seconds; within 1 minute, this synthesis was 60 times greater than that of controls. Thus, the initial increase in diacylglycerol is due to both increased hydrolysis of phospholipids and a burst of de novo phosphatidic acid synthesis. After 5 to 10 minutes, de novo phosphatidic acid synthesis continues as a major source of diacylglycerol. Both phospholipid effects of insulin seem important for generating diacylglycerol and other phospholipid-derived intracellular signaling substances.\n          </jats:p>","journal":"Science","year":1987,"id":688952,"datarank":0.7954957362088615,"base_score":5.303304908059076,"endowment":5.303304908059076,"self_citation_contribution":0.7954957362088615,"citation_network_contribution":0.0,"self_endowment_contribution":0.7954957362088615,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":200,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1799874,"name":"Thomas Suman Konda","orcid":null,"position":1,"is_corresponding":false},{"id":404258,"name":"John S. Davis","orcid":"0000-0003-3468-4079","position":2,"is_corresponding":false},{"id":1678344,"name":"Mary L. Standaert","orcid":null,"position":3,"is_corresponding":false},{"id":1799875,"name":"Robert J. Pollet","orcid":null,"position":4,"is_corresponding":false},{"id":1799876,"name":"Denise R. Cooper","orcid":null,"position":5,"is_corresponding":false},{"id":263113,"name":"Robert V. Farese","orcid":"0000-0001-8103-2239","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Insulin Rapidly Increases Diacylglycerol by Activating De Novo Phosphatidic Acid Synthesis","abstract":"<jats:p>\n            The mechanisms whereby insulin increases diacylglycerol in BC3H-1 myocytes were examined. When [\n            <jats:sup>3</jats:sup>\n            H]arachidonate labeling of phospholipids was used as an indicator of phospholipase C activation, transient increases in [\n            <jats:sup>3</jats:sup>\n            H]diacylglycerol were observed between 0.5 and 10 minutes after the onset of insulin treatment. With [\n            <jats:sup>3</jats:sup>\n            H]glycerol labeling as an indicator of de novo phospholipid synthesis, [\n            <jats:sup>3</jats:sup>\n            H]diacylglycerol was increased maximally at 1 minute and remained elevated for 20 minutes. [\n            <jats:sup>3</jats:sup>\n            H]Glycerol-labeled diacylglycerol was largely derived directly from phosphatidic acid. Insulin increased de novo phosphatidic acid synthesis within 5 to 10 seconds; within 1 minute, this synthesis was 60 times greater than that of controls. Thus, the initial increase in diacylglycerol is due to both increased hydrolysis of phospholipids and a burst of de novo phosphatidic acid synthesis. After 5 to 10 minutes, de novo phosphatidic acid synthesis continues as a major source of diacylglycerol. Both phospholipid effects of insulin seem important for generating diacylglycerol and other phospholipid-derived intracellular signaling substances.\n          </jats:p>","is_dataset_classified":null,"base_score":5.303304908059076,"endowment":5.303304908059076,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"3107122","pmcid":null,"openalex_id":"https://openalex.org/W2009553840","authors":[],"funders":[{"funder_name":"NICHD NIH HHS","grant_id":"HD22248","title":null},{"funder_name":"NIADDK NIH HHS","grant_id":"AM18608","title":null}],"total_grants":2,"fwci":30.2676,"citation_percentile":0.99865108,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2014,"count":1},{"year":2018,"count":1},{"year":2022,"count":1},{"year":2024,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://www.science.org/doi/pdf/10.1126/science.3107122","host_type":"publisher"},{"url":"https://doi.org/10.1126/science.3107122","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/3107122","host_type":"repository"}],"fields_of_study":["Adipose Tissue and Metabolism","Lipid metabolism and biosynthesis","Pancreatic function and diabetes","Arachidonic Acid","Arachidonic Acids","Cell Line","Diglycerides","Enzyme Activation","Glycerides","Glycerol","Insulin","Kinetics","Muscles","Phosphatidic Acids","Phosphatidylinositols","Phospholipids","Type C Phospholipases"],"mesh_terms":["Arachidonic Acids","Cell Line","Diglycerides","Enzyme Activation","Glycerides","Glycerol","Insulin","Kinetics","Muscles","Phosphatidic Acids","Phosphatidylinositols","Type C Phospholipases","Phospholipids","Arachidonic Acid"],"keywords":["Phosphatidic acid","Diacylglycerol kinase","Phospholipid","Diglyceride","Chemistry","Phospholipase D","Biochemistry","Intracellular","Second messenger system","Protein kinase C","Signal transduction","Enzyme"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-19T20:46:43.442802Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}