{"doi":"10.1126/science.2430332","title":"Efficient Trans-Splicing of a Yeast Mitochondrial RNA Group II Intron Implicates a Strong 5′ Exon-Intron Interaction","abstract":"<jats:p>The reaction mechanism for self-splicing introns requires the existence of a 5′ exon binding site on the intron. Experimental evidence is now presented consistent with the existence of such a binding site by demonstrating efficient and accurate trans-self-splicing of a yeast mitochondrial group II intron. Partial and complete trans-splicing reactions take place in the absence of branch formation, part of the usual pathway of nuclear splicing and group II self-splicing. In addition to indicating the existence of a 5′ exon binding site on the intron, the results have mechanistic implications for group II self-splicing and perhaps for nuclear splicing as well.</jats:p>","journal":"Science","year":1986,"id":33817,"datarank":5.143338493953168,"base_score":4.356708826689592,"endowment":4.356708826689592,"self_citation_contribution":0.6535063240034389,"citation_network_contribution":4.489832169949729,"self_endowment_contribution":0.6535063240034389,"citer_contribution":4.489832169949729,"corpus_percentile":null,"corpus_rank":null,"citation_count":77,"citer_count":69,"citers_with_citation_signal":62,"citers_with_endowment":62,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":176195,"name":"Michaek Rosbash","orcid":null,"position":1,"is_corresponding":false},{"id":175786,"name":"Alain Jacquier","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.356708826689592,"endowment":4.356708826689592,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2430332","pmcid":null,"openalex_id":"https://openalex.org/W1968177409","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM23549","title":null}],"total_grants":1,"fwci":3.8326,"citation_percentile":0.9416036,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2021,"count":2},{"year":2024,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://www.science.org/doi/pdf/10.1126/science.2430332","host_type":"publisher"},{"url":"https://doi.org/10.1126/science.2430332","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2430332","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","RNA modifications and cancer","RNA Research and Splicing","Biology","Medicine","Exons","Introns","Mitochondria","RNA","RNA Splicing","RNA, Mitochondrial","Saccharomyces cerevisiae","Tetrahymena"],"mesh_terms":["RNA, Mitochondrial","Exons","Introns","Mitochondria","RNA","RNA Splicing","Saccharomyces cerevisiae","Tetrahymena"],"keywords":["Intron","RNA splicing","Exon","Group II intron","Genetics","Biology","Mitochondrial DNA","Yeast","RNA","Group I catalytic intron","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T16:58:45.754535Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}