{"doi":"10.1126/science.1256773","title":"Spatial organization of cytokinesis signaling reconstituted in a cell-free system","abstract":"<jats:p>\n            During animal cell division, the cleavage furrow is positioned by microtubules that signal to the actin cortex at the cell midplane. We developed a cell-free system to recapitulate cytokinesis signaling using cytoplasmic extract from\n            <jats:italic>Xenopus</jats:italic>\n            eggs. Microtubules grew out as asters from artificial centrosomes and met to organize antiparallel overlap zones. These zones blocked the interpenetration of neighboring asters and recruited cytokinesis midzone proteins, including the chromosomal passenger complex (CPC) and centralspindlin. The CPC was transported to overlap zones, which required two motor proteins, Kif4A and a Kif20A paralog. Using supported lipid bilayers to mimic the plasma membrane, we observed the recruitment of cleavage furrow markers, including an active RhoA reporter, at microtubule overlaps. This system opens further approaches to understanding the biophysics of cytokinesis signaling.\n          </jats:p>","journal":"Science","year":2014,"id":42435,"datarank":4.230352236814779,"base_score":4.882801922586371,"endowment":4.882801922586371,"self_citation_contribution":0.7324202883879557,"citation_network_contribution":3.4979319484268236,"self_endowment_contribution":0.7324202883879557,"citer_contribution":3.4979319484268236,"corpus_percentile":null,"corpus_rank":null,"citation_count":131,"citer_count":111,"citers_with_citation_signal":96,"citers_with_endowment":96,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":203127,"name":"Aaron C. Groen","orcid":null,"position":1,"is_corresponding":false},{"id":203128,"name":"Martin Loose","orcid":null,"position":2,"is_corresponding":false},{"id":203129,"name":"Keisuke Ishihara","orcid":null,"position":3,"is_corresponding":false},{"id":203130,"name":"Martin Wühr","orcid":null,"position":4,"is_corresponding":false},{"id":203131,"name":"Christine M. Field","orcid":null,"position":5,"is_corresponding":false},{"id":201528,"name":"Timothy J. Mitchison","orcid":null,"position":6,"is_corresponding":false},{"id":203126,"name":"Phuong A. Nguyen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.882801922586371,"endowment":4.882801922586371,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25301629","pmcid":"PMC4281018","openalex_id":"https://openalex.org/W2030104183","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM039565","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R37 GM039565","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM103785","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM39565","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM103785","title":null}],"total_grants":5,"fwci":5.7879,"citation_percentile":0.96758543,"influential_citations":8,"citation_trend":[{"year":2014,"count":1},{"year":2015,"count":8},{"year":2016,"count":16},{"year":2017,"count":13},{"year":2018,"count":14},{"year":2019,"count":17},{"year":2020,"count":15},{"year":2021,"count":12},{"year":2022,"count":11},{"year":2023,"count":7},{"year":2024,"count":7},{"year":2025,"count":6},{"year":2026,"count":4}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4281018","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc4281018?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4281018","host_type":"repository"},{"url":"https://www.science.org/doi/pdf/10.1126/science.1256773","host_type":"publisher"},{"url":"https://doi.org/10.1126/science.1256773","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25301629","host_type":"repository"}],"fields_of_study":["Microtubule and mitosis dynamics","Photosynthetic Processes and Mechanisms","Cellular Mechanics and Interactions","Medicine","Biology","Animals","Cell Membrane","Cell-Free System","Centrosome","Cytokinesis","DNA-Binding Proteins","Guanosine Triphosphate","Kinesins","Lipid Bilayers","Microtubules","Models, Biological","Nuclear Proteins","Signal Transduction","Xenopus laevis","rhoA GTP-Binding Protein"],"mesh_terms":["Animals","Cell Membrane","Cell-Free System","DNA-Binding Proteins","Guanosine Triphosphate","Lipid Bilayers","Microtubules","Models, Biological","Nuclear Proteins","Xenopus laevis","Signal Transduction","Kinesins","Centrosome","rhoA GTP-Binding Protein","Cytokinesis"],"keywords":["Cytokinesis","Cleavage furrow","Cell biology","Cell cortex","Midbody","Centrosome","Microtubule","Biology","RHOA","Xenopus","Cell division","Signal transduction","Cytoskeleton","Cell","Cell cycle","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T02:58:39.666548Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}