{"doi":"10.1126/science.1221976","title":"Membrane Fusion Intermediates via Directional and Full Assembly of the SNARE Complex","abstract":"<jats:title>No More Fusion Confusion</jats:title>\n          <jats:p>\n            Biophysical models explain membrane fusion as a sequence of steps—including membrane contact, formation of a fusion stalk (merger of proximal monolayers), development of contact between distal monolayers that may or may not expand (hemifusion), and, finally, rupture of this diaphragm resulting in the opening of a fusion pore. Biological membrane fusion reactions are often driven by so-called SNARE proteins. By using a reconstituted membrane fusion system,\n            <jats:bold>\n              Hernandez\n              <jats:italic>et al.</jats:italic>\n            </jats:bold>\n            (p.\n            <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" page=\"1581\" related-article-type=\"in-this-issue\" vol=\"336\" xlink:href=\"10.1126/science.1221976\">1581</jats:related-article>\n            , published online 31 May) have now been able to correlate precisely the states of SNARE zippering with intermediate structures along the fusion pathway. The results suggest that a tightly docked state, with a membrane distance so close that no proteins fit in between them, represents a critical fusion intermediate as a consequence of SNARE zippering. This intermediate is incompatible with a SNARE-driven stalk or with a ringlike arrangement of SNAREs depicted in most current models of membrane fusion.\n          </jats:p>","journal":"Science","year":2012,"id":607743,"datarank":0.837524446317255,"base_score":5.583496308781699,"endowment":5.583496308781699,"self_citation_contribution":0.837524446317255,"citation_network_contribution":0.0,"self_endowment_contribution":0.837524446317255,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":265,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":226649,"name":"Alexander Stein","orcid":"0000-0002-9768-0086","position":1,"is_corresponding":false},{"id":137808,"name":"Elmar Behrmann","orcid":null,"position":2,"is_corresponding":false},{"id":247305,"name":"Dietmar Riedel","orcid":"0000-0003-2970-6894","position":3,"is_corresponding":false},{"id":1560632,"name":"Anna Cypionka","orcid":null,"position":4,"is_corresponding":false},{"id":1403133,"name":"Zohreh Farsi","orcid":"0000-0001-7274-981X","position":5,"is_corresponding":false},{"id":1560634,"name":"Peter J. Walla","orcid":null,"position":6,"is_corresponding":false},{"id":662309,"name":"Stefan Raunser","orcid":"0000-0001-9373-3016","position":7,"is_corresponding":false},{"id":228572,"name":"Reinhard Jahn","orcid":"0000-0003-1542-3498","position":8,"is_corresponding":false},{"id":1560631,"name":"Javier M. Hernandez","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Membrane Fusion Intermediates via Directional and Full Assembly of the SNARE Complex","abstract":"<jats:title>No More Fusion Confusion</jats:title>\n          <jats:p>\n            Biophysical models explain membrane fusion as a sequence of steps—including membrane contact, formation of a fusion stalk (merger of proximal monolayers), development of contact between distal monolayers that may or may not expand (hemifusion), and, finally, rupture of this diaphragm resulting in the opening of a fusion pore. Biological membrane fusion reactions are often driven by so-called SNARE proteins. By using a reconstituted membrane fusion system,\n            <jats:bold>\n              Hernandez\n              <jats:italic>et al.</jats:italic>\n            </jats:bold>\n            (p.\n            <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" page=\"1581\" related-article-type=\"in-this-issue\" vol=\"336\" xlink:href=\"10.1126/science.1221976\">1581</jats:related-article>\n            , published online 31 May) have now been able to correlate precisely the states of SNARE zippering with intermediate structures along the fusion pathway. The results suggest that a tightly docked state, with a membrane distance so close that no proteins fit in between them, represents a critical fusion intermediate as a consequence of SNARE zippering. This intermediate is incompatible with a SNARE-driven stalk or with a ringlike arrangement of SNAREs depicted in most current models of membrane fusion.\n          </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22653732","pmcid":"PMC3677693","openalex_id":null,"authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"3P01GM072694-05S1","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"P01 GM072694","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"other-oa","oa_locations":[{"url":"http://hdl.handle.net/11858/00-001M-0000-000F-A228-9","host_type":"repository"},{"url":"https://www.science.org/doi/pdf/10.1126/science.1221976","host_type":"publisher"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3677693","host_type":"repository"},{"url":"https://hdl.handle.net/11858/00-001M-0000-000F-A228-9","host_type":"repository"}],"fields_of_study":[],"mesh_terms":["Animals","Rats","Lipid Bilayers","Liposomes","Membrane Fusion","Protein Conformation","Protein Binding","SNARE Proteins","Vesicle-Associated Membrane Protein 2"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T06:57:06.166558Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}