{"doi":"10.1126/science.1175371","title":"Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions","abstract":"Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations have so far prevented a global analysis of lysine acetylation's cellular roles. We used high-resolution mass spectrometry to identify 3600 lysine acetylation sites on 1750 proteins and quantified acetylation changes in response to the deacetylase inhibitors suberoylanilide hydroxamic acid and MS-275. Lysine acetylation preferentially targets large macromolecular complexes involved in diverse cellular processes, such as chromatin remodeling, cell cycle, splicing, nuclear transport, and actin nucleation. Acetylation impaired phosphorylation-dependent interactions of 14-3-3 and regulated the yeast cyclin-dependent kinase Cdc28. Our data demonstrate that the regulatory scope of lysine acetylation is broad and comparable with that of other major posttranslational modifications.","journal":"Science","year":2009,"id":4803,"datarank":13.780665684598475,"base_score":8.299285906897275,"endowment":8.299285906897275,"self_citation_contribution":1.2448928860345914,"citation_network_contribution":12.535772798563883,"self_endowment_contribution":1.2448928860345914,"citer_contribution":12.535772798563883,"corpus_percentile":83.8,"corpus_rank":2394,"citation_count":4020,"citer_count":176,"citers_with_citation_signal":176,"citers_with_endowment":176,"datacite_reuse_total":0,"is_dataset":false,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2009-08-14","authors":[{"id":50218,"name":"Chanchal Kumar","orcid":null,"position":1,"is_corresponding":false},{"id":50219,"name":"Florian Gnad","orcid":"0000-0002-2287-2251","position":2,"is_corresponding":false},{"id":50220,"name":"Michael L. Nielsen","orcid":"0000-0002-0067-9039","position":3,"is_corresponding":false},{"id":50221,"name":"Michael Rehman","orcid":"0000-0002-2660-7682","position":4,"is_corresponding":false},{"id":50222,"name":"Tobias C. Walther","orcid":"0000-0003-1442-1327","position":5,"is_corresponding":false},{"id":50223,"name":"Jesper V. Olsen","orcid":"0000-0002-4747-4938","position":6,"is_corresponding":false},{"id":2937,"name":"Matthias Mann","orcid":"0000-0003-1292-4799","position":7,"is_corresponding":false},{"id":50217,"name":"Chunaram Choudhary","orcid":"0000-0002-9863-433X","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}