{"doi":"10.1126/science.1097931","title":"Dissection of the Mammalian Midbody Proteome Reveals Conserved Cytokinesis Mechanisms","abstract":"<jats:p>\n                    Cytokinesis is the essential process that partitions cellular contents into daughter cells. To identify and characterize cytokinesis proteins rapidly, we used a functional proteomic and comparative genomic strategy. Midbodies were isolated from mammalian cells, proteins were identified by multidimensional protein identification technology (MudPIT), and protein function was assessed in\n                    <jats:italic>Caenorhabditis elegans</jats:italic>\n                    . Of 172 homologs disrupted by RNA interference, 58% displayed defects in cleavage furrow formation or completion, or germline cytokinesis. Functional dissection of the midbody demonstrated the importance of lipid rafts and vesicle trafficking pathways in cytokinesis, and the utilization of common membrane cytoskeletal components in diverse morphogenetic events in the cleavage furrow, the germline, and neurons.\n                  </jats:p>","journal":"Science","year":2004,"id":42622,"datarank":13.211842097104425,"base_score":6.238324625039508,"endowment":6.238324625039508,"self_citation_contribution":0.9357486937559263,"citation_network_contribution":12.276093403348499,"self_endowment_contribution":0.9357486937559263,"citer_contribution":12.276093403348499,"corpus_percentile":null,"corpus_rank":null,"citation_count":511,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":8,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":66213,"name":"Hongbin Liu","orcid":"0000-0002-5840-2374","position":1,"is_corresponding":false},{"id":203768,"name":"John Yates","orcid":null,"position":2,"is_corresponding":false},{"id":203769,"name":"Barbara J. Meyer","orcid":null,"position":3,"is_corresponding":false},{"id":203770,"name":"Rebecca Heald","orcid":null,"position":4,"is_corresponding":false},{"id":201547,"name":"Ahna R. Skop","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":6.238324625039508,"endowment":6.238324625039508,"datacite_reuse_total":8,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15166316","pmcid":"PMC3679889","openalex_id":"https://openalex.org/W2080386975","authors":[],"funders":[{"funder_name":"NCRR NIH HHS","grant_id":"RR1823","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"F32 GM64159-01","title":null},{"funder_name":"NCRR NIH HHS","grant_id":"P41 RR011823","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"F32 GM064159","title":null}],"total_grants":4,"fwci":12.9805,"citation_percentile":0.99491831,"influential_citations":28,"citation_trend":[{"year":2012,"count":33},{"year":2013,"count":22},{"year":2014,"count":26},{"year":2015,"count":20},{"year":2016,"count":23},{"year":2017,"count":20},{"year":2018,"count":12},{"year":2019,"count":24},{"year":2020,"count":17},{"year":2021,"count":11},{"year":2022,"count":28},{"year":2023,"count":20},{"year":2024,"count":11},{"year":2025,"count":9},{"year":2026,"count":6}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://www.science.org/cms/asset/f67242ec-9f5f-4476-9b5c-c0e384ecd7fe/pap.pdf","host_type":"GREEN"},{"url":"https://www.science.org/doi/pdf/10.1126/science.1097931","host_type":"publisher"},{"url":"https://doi.org/10.1126/science.1097931","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15166316","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3679889","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Microtubule and mitosis dynamics","Endoplasmic Reticulum Stress and Disease","Biology","Medicine","Animals","CHO Cells","Caenorhabditis elegans","Carrier Proteins","Cell Cycle","Cell Division","Cell Fractionation","Cell Membrane","Computational Biology","Cricetinae","Cytoskeletal Proteins","Cytoskeleton","Germ Cells","HeLa Cells","Humans","Membrane Microdomains","Morphogenesis","Organelles","Protein Transport","Proteins","Proteome","Proteomics","Signal Transduction","Spindle Apparatus"],"mesh_terms":["Animals","Carrier Proteins","Cell Cycle","Cell Division","Cell Fractionation","Cell Membrane","Cytoskeletal Proteins","Cytoskeleton","Germ Cells","Cricetinae","HeLa Cells","Humans","Spindle Apparatus","Morphogenesis","Proteins","Organelles","Signal Transduction","CHO Cells","Caenorhabditis elegans","Computational Biology","Proteome","Protein Transport","Membrane Microdomains","Proteomics","Hela Cells"],"keywords":["Midbody","Cytokinesis","Cell biology","Proteome","Dissection (medical)","Biology","Septin","Computational biology","Genetics","Cell","Cell division","Anatomy"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.20428528.v1","title":"Additional file 6 of Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic apparatus","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.20428528","title":"Additional file 6 of Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic apparatus","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.20428531.v1","title":"Additional file 7 of Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic apparatus","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.20428531","title":"Additional file 7 of Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic 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apparatus","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T04:16:20.206742Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}