{"doi":"10.1126/sciadv.adz8096","title":"An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function","abstract":"Siglecs are immunomodulatory receptors that regulate immune cell function. A fundamental challenge in studying Siglec-ligand interactions is the low affinity of Siglecs for their ligands. Inspired by how nature uses multivalency, we developed Siglec-liposomes as a highly multivalent and versatile platform for detecting Siglec glycan ligands in which recombinant Siglecs were conjugated to liposomes using the SpyCatcher-SpyTag system. Siglec-liposomes offer tunable multivalency and a modular assembly, enabling presentation of different Siglecs on the same liposome. Using Siglec-liposomes, we profiled Siglec ligands on human leukocytes, revealing distinct patterns of Siglec ligands. Moreover, Siglec-liposomes are in vivo compatible, where we demonstrated that Siglec-7-liposomes bind to the brain vasculature in a mucin domain-dependent manner. Given the abundance of Siglec ligands on T cells, we investigated whether Siglec-liposomes modulate T cell function and find that Siglec-7-liposomes increase T cell proliferation in an ST3Gal1-dependent and CD43-independent manner. Together, Siglec-liposomes are a versatile and sensitive tool for detecting Siglec ligands and immunomodulation.","journal":"Science Advances","year":2025,"id":529319,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9499,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1227611,"name":"Edward N. Schmidt","orcid":"0000-0003-0729-5473","position":1,"is_corresponding":false},{"id":1408097,"name":"Madeline Crichton","orcid":"0009-0003-8783-7881","position":2,"is_corresponding":false},{"id":1408098,"name":"Kei Takahashi","orcid":"0000-0002-7925-6636","position":3,"is_corresponding":false},{"id":1408099,"name":"Guilherme Meira Lima","orcid":"0000-0002-3268-6081","position":4,"is_corresponding":false},{"id":1408100,"name":"Liany Luna-Dulcey","orcid":"0000-0002-5931-4456","position":5,"is_corresponding":false},{"id":1219750,"name":"Jaesoo Jung","orcid":"0000-0002-9247-1066","position":6,"is_corresponding":false},{"id":686052,"name":"Sabine Ivison","orcid":null,"position":7,"is_corresponding":false},{"id":1408101,"name":"Chris D. St. Laurent","orcid":"0009-0007-9500-7406","position":8,"is_corresponding":false},{"id":1408102,"name":"Jhon R. Enterina","orcid":"0000-0002-9137-3667","position":9,"is_corresponding":false},{"id":1408103,"name":"Sung-Yao Lin","orcid":"0009-0005-9252-0568","position":10,"is_corresponding":false},{"id":893468,"name":"Susmita Sarkar","orcid":null,"position":11,"is_corresponding":false},{"id":317194,"name":"Maju Joe","orcid":"0000-0003-0664-5275","position":12,"is_corresponding":false},{"id":1408608,"name":"Fahima Mozaneh","orcid":null,"position":13,"is_corresponding":false},{"id":1408104,"name":"Reni John","orcid":"0009-0003-5932-1378","position":14,"is_corresponding":false},{"id":284357,"name":"Som G. Nanjappa","orcid":"0000-0001-8022-1353","position":15,"is_corresponding":false},{"id":259930,"name":"Stacy A. Malaker","orcid":"0000-0003-2382-5067","position":16,"is_corresponding":false},{"id":685436,"name":"Megan K. Levings","orcid":"0000-0002-0305-5790","position":17,"is_corresponding":false},{"id":266074,"name":"Jamey D. Marth","orcid":"0000-0001-9323-1649","position":18,"is_corresponding":false},{"id":678345,"name":"Ratmir Derda","orcid":"0000-0003-1365-6570","position":19,"is_corresponding":false},{"id":296111,"name":"Matthew S. Macauley","orcid":"0000-0003-4579-1048","position":20,"is_corresponding":false},{"id":1374502,"name":"Zeinab Jame-Chenarboo","orcid":"0009-0003-4687-8652","position":0,"is_corresponding":true}],"reference_count":74,"raw_metadata":null,"created_at":"2026-07-19T02:50:56.971987Z","pmid":"41223255","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}