{"doi":"10.1126/sciadv.ads9443","title":"GTP hydrolysis triggers membrane remodeling by AMPH-1","abstract":"Membrane-enclosed transport carriers return biological molecules from the recycling endosome to the plasma membrane using a mechanism that is not well understood. In Caenorhabditis elegans , the formation of carriers from the recycling endosome requires the amphiphysin protein AMPH-1. Recently, we found that purified AMPH-1 is sufficient for tubulation and vesiculation of liposomes in a mechanism that is regulated by guanine nucleotides. Here, we propose a model linking guanosine 5′-triphosphate (GTP) binding and hydrolysis to the membrane binding and tubulation required for transport carrier formation. We find that GTP binding stabilizes interactions between AMPH-1 and the membrane through amphipathic, amino-terminal α helices, which are found at the tips of the arc-shaped, homodimeric structure. By contrast, in the posthydrolysis, guanosine diphosphate–bound state, these helices are repositioned to interact with the amino-terminal helices of other homodimers, to form an oligomeric AMPH-1 lattice that tubulates the membrane, in preparation for carrier formation by membrane fission.","journal":"Science Advances","year":2025,"id":571440,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9534,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":902836,"name":"Yuhang Wang","orcid":"0000-0002-8048-138X","position":1,"is_corresponding":false},{"id":1477583,"name":"Brianna Martin","orcid":null,"position":2,"is_corresponding":false},{"id":432232,"name":"Junjie Zhang","orcid":"0000-0002-7912-0819","position":3,"is_corresponding":false},{"id":978523,"name":"Chavela M. Carr","orcid":null,"position":4,"is_corresponding":false},{"id":486475,"name":"Hays S. Rye","orcid":"0000-0002-9420-3137","position":5,"is_corresponding":false},{"id":978133,"name":"Wei Gai","orcid":"0009-0005-5681-7380","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-19T02:57:15.755535Z","pmid":"40749062","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}