{"doi":"10.1126/sciadv.adj5539","title":"Eukaryotic Kv channel Shaker inactivates through selectivity filter dilation rather than collapse","abstract":"Eukaryotic voltage-gated K + channels have been extensively studied, but the structural bases for some of their most salient functional features remain to be established. C-type inactivation, for example, is an auto-inhibitory mechanism that confers temporal resolution to their signal-firing activity. In a recent breakthrough, studies of a mutant of Shaker that is prone to inactivate indicated that this process entails a dilation of the selectivity filter, the narrowest part of the ion conduction pathway. Here, we report an atomic-resolution cryo–electron microscopy structure that demonstrates that the wild-type channel can also adopt this dilated state. All-atom simulations corroborate this conformation is congruent with the electrophysiological characteristics of the C-type inactivated state, namely, residual K + conductance and altered ion specificity, and help rationalize why inactivation is accelerated or impeded by certain mutations. In summary, this study establishes the molecular basis for an important self-regulatory mechanism in eukaryotic K + channels, laying a solid foundation for further studies.","journal":"Science Advances","year":2023,"id":339063,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":253152,"name":"Xiao-Feng Tan","orcid":null,"position":1,"is_corresponding":false},{"id":806975,"name":"Chanhyung Bae","orcid":"0000-0003-3407-2857","position":2,"is_corresponding":false},{"id":806976,"name":"Ana I. Fernández-Mariño","orcid":"0000-0002-6995-4529","position":3,"is_corresponding":false},{"id":307889,"name":"Kenton J. Swartz","orcid":"0000-0003-3419-0765","position":4,"is_corresponding":false},{"id":251549,"name":"José D. Faraldo‐Gómez","orcid":"0000-0001-7224-7676","position":5,"is_corresponding":false},{"id":297245,"name":"Robyn Stix","orcid":"0000-0003-2128-7875","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-19T01:10:45.048445Z","pmid":"38064553","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}