{"doi":"10.1126/sciadv.adi9036","title":"ACD15, ACD21, and SLN regulate the accumulation and mobility of MBD6 to silence genes and transposable elements","abstract":"DNA methylation mediates silencing of transposable elements and genes in part via recruitment of the Arabidopsis MBD5/6 complex, which contains the methyl-CpG binding domain (MBD) proteins MBD5 and MBD6, and the J-domain containing protein SILENZIO (SLN). Here, we characterize two additional complex members: α-crystalline domain (ACD) containing proteins ACD15 and ACD21. We show that they are necessary for gene silencing, bridge SLN to the complex, and promote higher-order multimerization of MBD5/6 complexes within heterochromatin. These complexes are also highly dynamic, with the mobility of MBD5/6 complexes regulated by the activity of SLN. Using a dCas9 system, we demonstrate that tethering the ACDs to an ectopic site outside of heterochromatin can drive a massive accumulation of MBD5/6 complexes into large nuclear bodies. These results demonstrate that ACD15 and ACD21 are critical components of the gene-silencing MBD5/6 complex and act to drive the formation of higher-order, dynamic assemblies at CG methylation (meCG) sites.","journal":"Science Advances","year":2023,"id":337775,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9537,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":640211,"name":"Lucia Ichino","orcid":"0000-0002-0559-1626","position":1,"is_corresponding":false},{"id":872474,"name":"Shuya Wang","orcid":"0000-0001-9850-4634","position":2,"is_corresponding":false},{"id":658319,"name":"Jason Gardiner","orcid":"0000-0001-9387-6383","position":3,"is_corresponding":false},{"id":873221,"name":"Jaewon Yun","orcid":null,"position":4,"is_corresponding":false},{"id":344710,"name":"Yasaman Jami‐Alahmadi","orcid":"0000-0001-8289-2222","position":5,"is_corresponding":false},{"id":345447,"name":"Jihui Sha","orcid":"0000-0002-7358-1606","position":6,"is_corresponding":false},{"id":1070231,"name":"Cristy P. Mendoza","orcid":null,"position":7,"is_corresponding":false},{"id":1069721,"name":"Bailey J. Steelman","orcid":"0009-0006-2429-9854","position":8,"is_corresponding":false},{"id":1069722,"name":"Aliya van Aardenne","orcid":"0009-0007-9446-957X","position":9,"is_corresponding":false},{"id":1070232,"name":"Sophia Kira-Lucas","orcid":null,"position":10,"is_corresponding":false},{"id":1069723,"name":"Isabelle Trentchev","orcid":"0009-0000-4023-394X","position":11,"is_corresponding":false},{"id":14958,"name":"James A. Wohlschlegel","orcid":"0000-0003-3399-901X","position":12,"is_corresponding":false},{"id":225877,"name":"Steven E. Jacobsen","orcid":"0000-0001-9483-138X","position":13,"is_corresponding":false},{"id":271912,"name":"Brandon A. Boone","orcid":"0000-0002-6475-8000","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T01:10:31.133446Z","pmid":"37967186","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}