{"doi":"10.1126/sciadv.adi3647","title":"ATP1A3 as a target for isolating neuron-specific extracellular vesicles from human brain and biofluids","abstract":"Neuron-derived extracellular vesicles (NDEVs) are potential biomarkers of neurological diseases although their reliable molecular target is not well established. Here, we demonstrate that ATPase Na + /K + transporting subunit alpha 3 (ATP1A3) is abundantly expressed in extracellular vesicles (EVs) isolated from induced human neuron, brain, cerebrospinal fluid, and plasma in comparison with the presumed NDEV markers NCAM1 and L1CAM by using super-resolution microscopy and biochemical assessments. Proteomic analysis of immunoprecipitated ATP1A3 + brain-derived EVs shows higher enrichment of synaptic markers and cargo proteins relevant to Alzheimer’s disease (AD) compared to NCAM1 + or LICAM + EVs. Single particle analysis shows the elevated amyloid-β positivity in ATP1A3 + EVs from AD plasma, providing better diagnostic prediction of AD over other plasma biomarkers. Thus, ATP1A3 is a reliable target to isolate NDEV from biofluids for diagnostic research.","journal":"Science Advances","year":2023,"id":317037,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":86,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9611,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":493567,"name":"Zhengrong Zhang","orcid":"0000-0002-0608-8520","position":1,"is_corresponding":false},{"id":532121,"name":"Nadia Sultana","orcid":"0000-0001-8627-101X","position":2,"is_corresponding":false},{"id":18916,"name":"Maria Ericsson","orcid":"0000-0002-4563-3683","position":3,"is_corresponding":false},{"id":230884,"name":"Yuka A. Martens","orcid":"0000-0002-5194-4269","position":4,"is_corresponding":false},{"id":715714,"name":"Min Sun","orcid":"0000-0003-4389-9880","position":5,"is_corresponding":false},{"id":230892,"name":"Takahisa Kanekiyo","orcid":"0000-0001-6751-9374","position":6,"is_corresponding":false},{"id":236491,"name":"Seiko Ikezu","orcid":"0000-0001-9879-730X","position":7,"is_corresponding":false},{"id":109685,"name":"Scott A. Shaffer","orcid":"0000-0002-5264-3257","position":8,"is_corresponding":false},{"id":236493,"name":"Tsuneya Ikezu","orcid":"0000-0002-3979-8596","position":9,"is_corresponding":false},{"id":247311,"name":"Yang You","orcid":"0000-0003-0574-7827","position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":null,"created_at":"2026-07-19T01:06:44.698773Z","pmid":"37713494","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}