{"doi":"10.1126/sciadv.adi1562","title":"SYCP1 head-to-head assembly is required for chromosome synapsis in mouse meiosis","abstract":"<jats:p>\n            In almost all sexually reproducing organisms, meiotic recombination and cell division require the synapsis of homologous chromosomes by a large proteinaceous structure, the synaptonemal complex (SC). While the SC’s overall structure is highly conserved across eukaryotes, its constituent proteins diverge between phyla. Transverse filament protein, SYCP1, spans the width of the SC and undergoes amino-terminal head-to-head self-assembly in vitro through a motif that is unusually highly conserved across kingdoms of life. Here, we report creation of mouse mutants,\n            <jats:italic>\n              Sycp1\n              <jats:sup>L102E</jats:sup>\n            </jats:italic>\n            and\n            <jats:italic>\n              Sycp1\n              <jats:sup>L106E</jats:sup>\n            </jats:italic>\n            , that target SYCP1’s head-to-head interface. L106E resulted in a complete loss of synapsis, while L102E had no apparent effect on synapsis, in agreement with their differential effects on the SYCP1 head-to-head interface in molecular dynamics simulations. In\n            <jats:italic>\n              Sycp1\n              <jats:sup>L106E</jats:sup>\n            </jats:italic>\n            mice, homologs aligned and recruited low levels of mutant SYCP1 and other SC proteins, but the absence of synapsis led to failure of crossover formation and meiotic arrest. We conclude that SYCP1’s conserved head-to-head interface is essential for meiotic chromosome synapsis in vivo.\n          </jats:p>","journal":"Science Advances","year":2023,"id":593988,"datarank":0.4887144807032224,"base_score":3.258096538021482,"endowment":3.258096538021482,"self_citation_contribution":0.4887144807032224,"citation_network_contribution":0.0,"self_endowment_contribution":0.4887144807032224,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1520337,"name":"Emily A. Kesler","orcid":null,"position":1,"is_corresponding":false},{"id":560927,"name":"Dai Tsuchiya","orcid":"0000-0001-8385-2775","position":2,"is_corresponding":false},{"id":986427,"name":"Timothy J. Corbin","orcid":"0009-0008-6850-6805","position":3,"is_corresponding":false},{"id":1055025,"name":"Kyle Weaver","orcid":"0000-0002-3960-9177","position":4,"is_corresponding":false},{"id":265257,"name":"Andrea Moran","orcid":null,"position":5,"is_corresponding":false},{"id":376552,"name":"Zulin Yu","orcid":"0000-0002-1292-8426","position":6,"is_corresponding":false},{"id":1520338,"name":"Lane Adams","orcid":"0009-0005-1869-739X","position":7,"is_corresponding":false},{"id":1055027,"name":"Kym Delventhal","orcid":"0000-0003-0505-9792","position":8,"is_corresponding":false},{"id":1520339,"name":"Michael Durnin","orcid":null,"position":9,"is_corresponding":false},{"id":316418,"name":"Owen R. Davies","orcid":"0000-0002-3806-5403","position":10,"is_corresponding":false},{"id":410852,"name":"R. Scott Hawley","orcid":"0000-0002-6478-0494","position":11,"is_corresponding":false},{"id":526637,"name":"Katherine Kretovich Billmyre","orcid":"0000-0003-1681-9849","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"SYCP1 head-to-head assembly is required for chromosome synapsis in mouse meiosis","abstract":"<jats:p>\n            In almost all sexually reproducing organisms, meiotic recombination and cell division require the synapsis of homologous chromosomes by a large proteinaceous structure, the synaptonemal complex (SC). While the SC’s overall structure is highly conserved across eukaryotes, its constituent proteins diverge between phyla. Transverse filament protein, SYCP1, spans the width of the SC and undergoes amino-terminal head-to-head self-assembly in vitro through a motif that is unusually highly conserved across kingdoms of life. Here, we report creation of mouse mutants,\n            <jats:italic>\n              Sycp1\n              <jats:sup>L102E</jats:sup>\n            </jats:italic>\n            and\n            <jats:italic>\n              Sycp1\n              <jats:sup>L106E</jats:sup>\n            </jats:italic>\n            , that target SYCP1’s head-to-head interface. L106E resulted in a complete loss of synapsis, while L102E had no apparent effect on synapsis, in agreement with their differential effects on the SYCP1 head-to-head interface in molecular dynamics simulations. In\n            <jats:italic>\n              Sycp1\n              <jats:sup>L106E</jats:sup>\n            </jats:italic>\n            mice, homologs aligned and recruited low levels of mutant SYCP1 and other SC proteins, but the absence of synapsis led to failure of crossover formation and meiotic arrest. We conclude that SYCP1’s conserved head-to-head interface is essential for meiotic chromosome synapsis in vivo.\n          </jats:p>","is_dataset_classified":null,"base_score":3.2188758248682006,"endowment":3.2188758248682006,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37862414","pmcid":"PMC10588951","openalex_id":"https://openalex.org/W4387823519","authors":[],"funders":[{"funder_name":"Wellcome Trust","grant_id":"219413","title":"Molecular basis of chromosome synapsis and genetic exchange in mammalian meiosis"},{"funder_name":"Wellcome Trust","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null}],"total_grants":3,"fwci":2.2587,"citation_percentile":0.89181595,"influential_citations":0,"citation_trend":[{"year":2024,"count":8},{"year":2025,"count":8},{"year":2026,"count":8}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.adi1562?download=true","host_type":"journal"},{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.adi1562?download=true","host_type":"publisher"},{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.adi1562","host_type":"publisher"},{"url":"https://doi.org/10.1126/sciadv.adi1562","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37862414","host_type":"repository"},{"url":"https://www.research.ed.ac.uk/en/publications/fb4c32e8-ee63-4147-a5e5-78247b9e3316","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10588951","host_type":"repository"},{"url":"https://hdl.handle.net/20.500.11820/fb4c32e8-ee63-4147-a5e5-78247b9e3316","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10588951/pdf/sciadv.adi1562.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10588951","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10588951?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1126/sciadv.adi1562","host_type":""},{"url":"https://www.pure.ed.ac.uk/ws/files/388424850/sciadv.adi1562.pdf","host_type":""}],"fields_of_study":["DNA Repair Mechanisms","Genomics and Chromatin Dynamics","Epigenetics and DNA Methylation","0301 basic medicine","03 medical and health sciences","Animals","Mice","Chromosome Pairing","Homologous Recombination","Meiosis","Nuclear Proteins","Synaptonemal Complex","DNA-Binding Proteins"],"mesh_terms":["Animals","DNA-Binding Proteins","Meiosis","Nuclear Proteins","Synaptonemal Complex","Chromosome Pairing","Mice","Homologous Recombination"],"keywords":["Synapsis","Synaptonemal complex","Meiosis","Biology","Homologous chromosome","Genetics","Chromosome segregation","Cell biology","Mutant","Chromosomal crossover","Chromosome","Gene","DNA-Binding Proteins","Mice","Chromosome Pairing","Animals","Nuclear Proteins","Biomedicine and Life Sciences","Homologous Recombination"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T13:05:07.076709Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}