{"doi":"10.1126/sciadv.adg7545","title":"Discovery and verification of extracellular microRNA biomarkers for diagnostic and prognostic assessment of preeclampsia at triage","abstract":"We report on the identification of extracellular miRNA (ex-miRNA) biomarkers for early diagnosis and prognosis of preeclampsia (PE). Small RNA sequencing of maternal serum prospectively collected from participants undergoing evaluation for suspected PE revealed distinct patterns of ex-miRNA expression among different categories of hypertensive disorders in pregnancy. Applying an iterative machine learning method identified three bivariate miRNA biomarkers ( miR-522-3p/miR-4732-5p , miR-516a-5p/miR-144-3p , and miR-27b-3p/let-7b-5p ) that, when applied serially, distinguished between PE cases of different severity and differentiated cases from controls with a sensitivity of 93%, specificity of 79%, positive predictive value (PPV) of 55%, and negative predictive value (NPV) of 89%. In a small independent validation cohort, these ex-miRNA biomarkers had a sensitivity of 91% and specificity of 57%. Combining these ex-miRNA biomarkers with the established sFlt1:PlGF protein biomarker ratio performed better than either set of biomarkers alone (sensitivity of 89.4%, specificity of 91.3%, PPV of 95.5%, and NPV of 80.8%).","journal":"Science Advances","year":2023,"id":339100,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9583,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":347106,"name":"Lara Poling","orcid":"0000-0002-9145-7254","position":1,"is_corresponding":false},{"id":257529,"name":"Srimeenakshi Srinivasan","orcid":"0000-0002-5045-7880","position":2,"is_corresponding":false},{"id":1072660,"name":"Carolina Martinez-King","orcid":"0009-0003-3618-7997","position":3,"is_corresponding":false},{"id":1073187,"name":"Adanna Anyikam","orcid":null,"position":4,"is_corresponding":false},{"id":782448,"name":"Kathy Zhang‐Rutledge","orcid":"0000-0002-0534-2857","position":5,"is_corresponding":false},{"id":349207,"name":"Cuong To","orcid":null,"position":6,"is_corresponding":false},{"id":805353,"name":"Abbas Hakim","orcid":"0000-0002-2875-4715","position":7,"is_corresponding":false},{"id":1073188,"name":"Marina Mochizuki","orcid":null,"position":8,"is_corresponding":false},{"id":1072661,"name":"Kajal Verma","orcid":"0000-0001-9159-9722","position":9,"is_corresponding":false},{"id":1073189,"name":"Antoinette Mason","orcid":null,"position":10,"is_corresponding":false},{"id":347107,"name":"Vy Tran","orcid":"0009-0007-0516-7191","position":11,"is_corresponding":false},{"id":347108,"name":"Morgan Meads","orcid":"0000-0002-5970-7356","position":12,"is_corresponding":false},{"id":726116,"name":"Leah Lamale‐Smith","orcid":"0000-0003-0572-3980","position":13,"is_corresponding":false},{"id":349215,"name":"Hilary Roeder","orcid":null,"position":14,"is_corresponding":false},{"id":304371,"name":"Mariko Horii","orcid":"0000-0002-4996-6792","position":15,"is_corresponding":false},{"id":1072662,"name":"Gladys Ramos","orcid":"0000-0002-9838-4991","position":16,"is_corresponding":false},{"id":886856,"name":"Peter De Hoff","orcid":"0000-0002-0163-7535","position":17,"is_corresponding":false},{"id":295434,"name":"Mana M. Parast","orcid":"0000-0001-5963-2246","position":18,"is_corresponding":false},{"id":519964,"name":"Priyadarshini Pantham","orcid":null,"position":19,"is_corresponding":false},{"id":105299,"name":"Louise C. Laurent","orcid":"0000-0002-2095-7534","position":20,"is_corresponding":false},{"id":667811,"name":"Robert Morey","orcid":"0000-0001-8480-4564","position":0,"is_corresponding":true}],"reference_count":60,"raw_metadata":null,"created_at":"2026-07-19T01:10:45.048445Z","pmid":"38117879","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}