{"doi":"10.1126/sciadv.adg6693","title":"A MYCN-independent mechanism mediating secretome reprogramming and metastasis in <i>MYCN</i> -amplified neuroblastoma","abstract":"MYCN amplification ( MNA ) is a defining feature of high-risk neuroblastoma (NB) and predicts poor prognosis. However, whether genes within or in close proximity to the MYCN amplicon also contribute to MNA + NB remains poorly understood. Here, we identify that GREB1 , a transcription factor encoding gene neighboring the MYCN locus, is frequently coexpressed with MYCN and promotes cell survival in MNA + NB. GREB1 controls gene expression independently of MYCN, among which we uncover myosin 1B ( MYO1B ) as being highly expressed in MNA + NB and, using a chick chorioallantoic membrane (CAM) model, as a crucial regulator of invasion and metastasis. Global secretome and proteome profiling further delineates MYO1B in regulating secretome reprogramming in MNA + NB cells, and the cytokine MIF as an important pro-invasive and pro-metastatic mediator of MYO1B activity. Together, we have identified a putative GREB1-MYO1B-MIF axis as an unconventional mechanism promoting aggressive behavior in MNA + NB and independently of MYCN.","journal":"Science Advances","year":2023,"id":371541,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9587,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":240481,"name":"Alberto Delaidelli","orcid":"0000-0001-7416-6503","position":1,"is_corresponding":false},{"id":1131539,"name":"Sumreen Javed","orcid":"0000-0001-9120-3580","position":2,"is_corresponding":false},{"id":1131540,"name":"Busra Turgu","orcid":"0000-0002-0207-1115","position":3,"is_corresponding":false},{"id":1132098,"name":"Taylor Morrison","orcid":null,"position":4,"is_corresponding":false},{"id":562934,"name":"Christopher S. Hughes","orcid":"0000-0002-6980-4459","position":5,"is_corresponding":false},{"id":1132099,"name":"Xiaqiu Yang","orcid":null,"position":6,"is_corresponding":false},{"id":361041,"name":"Manideep C. Pachva","orcid":"0009-0002-9509-1613","position":7,"is_corresponding":false},{"id":634641,"name":"Michael M. Lizardo","orcid":"0000-0003-3981-0978","position":8,"is_corresponding":false},{"id":970938,"name":"Gurdeep Singh","orcid":"0000-0003-2519-0456","position":9,"is_corresponding":false},{"id":1131541,"name":"Jennifer Hoffmann","orcid":"0000-0002-5202-0893","position":10,"is_corresponding":false},{"id":266189,"name":"Yue Z. Huang","orcid":"0000-0002-3381-9640","position":11,"is_corresponding":false},{"id":550692,"name":"Khushbu Patel","orcid":"0000-0002-3269-7442","position":12,"is_corresponding":false},{"id":366508,"name":"Rawan Shraim","orcid":"0009-0005-5350-3386","position":13,"is_corresponding":false},{"id":634645,"name":"Sonia H.Y. Kung","orcid":"0000-0002-9230-8939","position":14,"is_corresponding":false},{"id":22222,"name":"Gregg B. Morin","orcid":"0000-0001-8949-4374","position":15,"is_corresponding":false},{"id":14317,"name":"Samuel Aparício","orcid":"0000-0002-0487-9599","position":16,"is_corresponding":false},{"id":29533,"name":"Daniel Martínez","orcid":"0000-0002-4723-9989","position":17,"is_corresponding":false},{"id":240511,"name":"John M. Maris","orcid":"0000-0002-8088-7929","position":18,"is_corresponding":false},{"id":225129,"name":"Kristopher R. Bosse","orcid":"0000-0002-1424-2858","position":19,"is_corresponding":false},{"id":1037899,"name":"Karla C. Williams","orcid":"0000-0003-2954-2869","position":20,"is_corresponding":false},{"id":240512,"name":"Poul H. Sorensen","orcid":"0000-0001-7447-2199","position":21,"is_corresponding":false},{"id":1061240,"name":"Hai‐Feng Zhang","orcid":null,"position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":null,"created_at":"2026-07-19T01:15:49.453761Z","pmid":"37611092","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}