{"doi":"10.1126/sciadv.adf2245","title":"Female naïve human pluripotent stem cells carry X chromosomes with Xa-like and Xi-like folding conformations","abstract":"Three-dimensional (3D) genomics shows immense promise for studying X chromosome inactivation (XCI) by interrogating changes to the X chromosomes’ 3D states. Here, we sought to characterize the 3D state of the X chromosome in naïve and primed human pluripotent stem cells (hPSCs). Using chromatin tracing, we analyzed X chromosome folding conformations in these cells with megabase genomic resolution. X chromosomes in female naïve hPSCs exhibit folding conformations similar to the active X chromosome (Xa) and the inactive X chromosome (Xi) in somatic cells. However, naïve X chromosomes do not exhibit the chromatin compaction typically associated with these somatic X chromosome states. In H7 naïve human embryonic stem cells, XIST accumulation observed on damaged X chromosomes demonstrates the potential for naïve hPSCs to activate XCI-related mechanisms. Overall, our findings provide insight into the X chromosome status of naïve hPSCs with a single-chromosome resolution and are critical in understanding the unique epigenetic regulation in early embryonic cells.","journal":"Science Advances","year":2023,"id":364313,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9413,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":270679,"name":"Bing Yang","orcid":"0000-0002-9355-2538","position":1,"is_corresponding":false},{"id":237105,"name":"Yoshiaki Tanaka","orcid":"0000-0002-2078-9374","position":2,"is_corresponding":false},{"id":289106,"name":"Kun‐Yong Kim","orcid":"0000-0001-8968-0177","position":3,"is_corresponding":false},{"id":845650,"name":"Bilal Çakir","orcid":"0000-0003-4610-0317","position":4,"is_corresponding":false},{"id":237106,"name":"Yangfei Xiang","orcid":"0000-0002-6256-6312","position":5,"is_corresponding":false},{"id":656575,"name":"Jonghun Kim","orcid":"0000-0002-9154-5529","position":6,"is_corresponding":false},{"id":270684,"name":"Siyuan Wang","orcid":"0000-0001-6550-4064","position":7,"is_corresponding":false},{"id":16529,"name":"In‐Hyun Park","orcid":"0000-0001-7748-1293","position":8,"is_corresponding":false},{"id":1119111,"name":"Benjamin Patterson","orcid":"0009-0008-4132-9407","position":0,"is_corresponding":true}],"reference_count":84,"raw_metadata":null,"created_at":"2026-07-19T01:14:36.728255Z","pmid":"37540754","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}