{"doi":"10.1126/sciadv.ade2727","title":"Subnanometer structure of an enveloped virus fusion complex on viral surface reveals new entry mechanisms","abstract":"Paramyxoviruses-including important pathogens like parainfluenza, measles, and Nipah viruses-use a receptor binding protein [hemagglutinin-neuraminidase (HN) for parainfluenza] and a fusion protein (F), acting in a complex, to enter cells. We use cryo-electron tomography to visualize the fusion complex of human parainfluenza virus 3 (HN/F) on the surface of authentic clinical viruses at a subnanometer resolution sufficient to answer mechanistic questions. An HN loop inserts in a pocket on F, showing how the fusion complex remains in a ready but quiescent state until activation. The globular HN heads are rotated with respect to each other: one downward to contact F, and the other upward to grapple cellular receptors, demonstrating how HN/F performs distinct steps before F activation. This depiction of viral fusion illuminates potentially druggable targets for paramyxoviruses and sheds light on fusion processes that underpin wide-ranging biological processes but have not been visualized in situ or at the present resolution.","journal":"Science Advances","year":2023,"id":322932,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":562794,"name":"Gillian Zipursky","orcid":"0000-0003-4408-5168","position":1,"is_corresponding":false},{"id":1037146,"name":"Wenjing Cheng","orcid":"0000-0002-9241-3835","position":2,"is_corresponding":false},{"id":622693,"name":"Kyle Stearns","orcid":"0000-0002-5166-7927","position":3,"is_corresponding":false},{"id":1037147,"name":"Shari Stenglein","orcid":"0000-0001-8894-509X","position":4,"is_corresponding":false},{"id":1037148,"name":"Kate Golub","orcid":"0000-0003-3350-9762","position":5,"is_corresponding":false},{"id":1037607,"name":"Frances Cohen","orcid":null,"position":6,"is_corresponding":false},{"id":225930,"name":"Francesca T. Bovier","orcid":"0000-0003-3446-9619","position":7,"is_corresponding":false},{"id":1037149,"name":"Daniel Pfalmer","orcid":"0000-0001-5896-5866","position":8,"is_corresponding":false},{"id":108342,"name":"Alexander L. Greninger","orcid":"0000-0002-7443-0527","position":9,"is_corresponding":false},{"id":225940,"name":"Matteo Porotto","orcid":"0000-0003-3866-9220","position":10,"is_corresponding":false},{"id":275518,"name":"Amédée des Georges","orcid":"0000-0002-9704-3781","position":11,"is_corresponding":false},{"id":225939,"name":"Anne Moscona","orcid":"0000-0002-1796-8320","position":12,"is_corresponding":false},{"id":395043,"name":"Tara C. Marcink","orcid":"0000-0003-4295-1229","position":0,"is_corresponding":true}],"reference_count":86,"raw_metadata":null,"created_at":"2026-07-19T01:07:47.432636Z","pmid":"36763666","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}