{"doi":"10.1126/sciadv.ade0090","title":"Topological screen identifies hundreds of Cp190- and CTCF-dependent\n                    <i>Drosophila</i>\n                    chromatin insulator elements","abstract":"<jats:p>\n                    <jats:italic>Drosophila</jats:italic>\n                    insulators were the first DNA elements found to regulate gene expression by delimiting chromatin contacts. We still do not know how many of them exist and what impact they have on the\n                    <jats:italic>Drosophila</jats:italic>\n                    genome folding. Contrary to vertebrates, there is no evidence that fly insulators block cohesin-mediated chromatin loop extrusion. Therefore, their mechanism of action remains uncertain. To bridge these gaps, we mapped chromatin contacts in\n                    <jats:italic>Drosophila</jats:italic>\n                    cells lacking the key insulator proteins CTCF and Cp190. With this approach, we found hundreds of insulator elements. Their study indicates that\n                    <jats:italic>Drosophila</jats:italic>\n                    insulators play a minor role in the overall genome folding but affect chromatin contacts locally at many loci. Our observations argue that Cp190 promotes cobinding of other insulator proteins and that the model, where\n                    <jats:italic>Drosophila</jats:italic>\n                    insulators block chromatin contacts by forming loops, needs revision. Our insulator catalog provides an important resource to study mechanisms of genome folding.\n                  </jats:p>","journal":"Science Advances","year":2023,"id":663056,"datarank":0.5533319181170905,"base_score":3.6888794541139363,"endowment":3.6888794541139363,"self_citation_contribution":0.5533319181170905,"citation_network_contribution":0.0,"self_endowment_contribution":0.5533319181170905,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":39,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1731049,"name":"Mikhail Savitsky","orcid":"0000-0002-4735-5517","position":1,"is_corresponding":false},{"id":1731050,"name":"Chikuan Kuong","orcid":"0000-0002-7459-5161","position":2,"is_corresponding":false},{"id":1731051,"name":"Caroline Jacquier","orcid":null,"position":3,"is_corresponding":false},{"id":65523,"name":"Giacomo Cavalli","orcid":"0000-0003-3709-3469","position":4,"is_corresponding":false},{"id":1731052,"name":"Jia-Ming Chang","orcid":"0000-0002-6711-1739","position":5,"is_corresponding":false},{"id":23401,"name":"Yuri B. Schwartz","orcid":"0000-0003-4790-3920","position":6,"is_corresponding":false},{"id":1731048,"name":"Tatyana G. Kahn","orcid":"0000-0001-6109-6243","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Topological screen identifies hundreds of Cp190- and CTCF-dependent\n                    <i>Drosophila</i>\n                    chromatin insulator elements","abstract":"<jats:p>\n                    <jats:italic>Drosophila</jats:italic>\n                    insulators were the first DNA elements found to regulate gene expression by delimiting chromatin contacts. We still do not know how many of them exist and what impact they have on the\n                    <jats:italic>Drosophila</jats:italic>\n                    genome folding. Contrary to vertebrates, there is no evidence that fly insulators block cohesin-mediated chromatin loop extrusion. Therefore, their mechanism of action remains uncertain. To bridge these gaps, we mapped chromatin contacts in\n                    <jats:italic>Drosophila</jats:italic>\n                    cells lacking the key insulator proteins CTCF and Cp190. With this approach, we found hundreds of insulator elements. Their study indicates that\n                    <jats:italic>Drosophila</jats:italic>\n                    insulators play a minor role in the overall genome folding but affect chromatin contacts locally at many loci. Our observations argue that Cp190 promotes cobinding of other insulator proteins and that the model, where\n                    <jats:italic>Drosophila</jats:italic>\n                    insulators block chromatin contacts by forming loops, needs revision. Our insulator catalog provides an important resource to study mechanisms of genome folding.\n                  </jats:p>","is_dataset_classified":null,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36735780","pmcid":"PMC9897668","openalex_id":"https://openalex.org/W4319069365","authors":[],"funders":[],"total_grants":0,"fwci":3.5242,"citation_percentile":0.9409815,"influential_citations":0,"citation_trend":[{"year":2023,"count":7},{"year":2024,"count":14},{"year":2025,"count":11},{"year":2026,"count":6}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.ade0090?download=true","host_type":"journal"},{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.ade0090?download=true","host_type":"publisher"},{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.ade0090","host_type":"publisher"},{"url":"https://doi.org/10.1126/sciadv.ade0090","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36735780","host_type":"repository"},{"url":"http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-204742","host_type":"repository"},{"url":"https://hal.umontpellier.fr/hal-04114124","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9897668","host_type":"repository"},{"url":"https://umu.diva-portal.org/smash/get/diva2:1738500/FULLTEXT01","host_type":"repository"},{"url":"https://hal.umontpellier.fr/hal-04114124/document","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9897668/pdf/sciadv.ade0090.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9897668","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9897668?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Genomics and Chromatin Dynamics","Chromosomal and Genetic Variations","RNA Research and Splicing","Animals","Drosophila","Chromatin","DNA-Binding Proteins","Drosophila Proteins","Insulator Elements","Nuclear Proteins","Microtubule-Associated Proteins","CCCTC-Binding Factor"],"mesh_terms":["CCCTC-Binding Factor","Animals","Chromatin","DNA-Binding Proteins","Drosophila","Microtubule-Associated Proteins","Nuclear Proteins","Drosophila Proteins","Insulator Elements"],"keywords":["CTCF","Chromatin","Insulator (electricity)","Drosophila melanogaster","Biology","Genome","Cohesin","Cell biology","Genetics","Gene","Computational biology","Physics","Gene expression","Optoelectronics","Enhancer"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"geo"},{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T19:24:46.981123Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}