{"doi":"10.1126/sciadv.add8659","title":"In situ visualization of Braun’s lipoprotein on\n            <i>E. coli</i>\n            sacculi","abstract":"<jats:p>\n            Braun’s lipoprotein (Lpp) plays a major role in stabilizing the integrity of the cell envelope in\n            <jats:italic>Escherichia coli</jats:italic>\n            , as it provides a covalent cross-link between the outer membrane and the peptidoglycan layer. An important challenge in elucidating the physiological role of Lpp lies in attaining a detailed understanding of its distribution on the peptidoglycan layer. Here, using atomic force microscopy, we visualized Lpp directly on peptidoglycan sacculi. Lpp is homogeneously distributed over the outer surface of the sacculus at a high density. However, it is absent at the constriction site during cell division, revealing its role in the cell division process with Pal, another cell envelope–associated protein. Collectively, we have established a framework to elucidate the distribution of Lpp and other peptidoglycan-bound proteins via a direct imaging modality.\n          </jats:p>","journal":"Science Advances","year":2023,"id":652932,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1703499,"name":"Meng-Yao Zhang","orcid":"0000-0002-5707-8453","position":1,"is_corresponding":false},{"id":1703500,"name":"Si-Min Liu","orcid":"0000-0003-2945-3321","position":2,"is_corresponding":false},{"id":1703501,"name":"Zhuo-Wei Chen","orcid":null,"position":3,"is_corresponding":false},{"id":1703502,"name":"Pei-Ling Yang","orcid":null,"position":4,"is_corresponding":false},{"id":1703503,"name":"Hong-Su Zhang","orcid":null,"position":5,"is_corresponding":false},{"id":1703504,"name":"Meng-Yun Liu","orcid":null,"position":6,"is_corresponding":false},{"id":1221951,"name":"Kang Li","orcid":"0000-0002-9141-860X","position":7,"is_corresponding":false},{"id":1703505,"name":"Long-Sheng Zhao","orcid":null,"position":8,"is_corresponding":false},{"id":1703506,"name":"Ning-Hua Liu","orcid":null,"position":9,"is_corresponding":false},{"id":1221953,"name":"Lu‐Ning Liu","orcid":"0000-0002-8884-4819","position":10,"is_corresponding":false},{"id":1703507,"name":"Xiu-Lan Chen","orcid":null,"position":11,"is_corresponding":false},{"id":809259,"name":"Jamie K. Hobbs","orcid":"0000-0002-5872-1404","position":12,"is_corresponding":false},{"id":501071,"name":"Simon J. Foster","orcid":"0000-0001-7432-7805","position":13,"is_corresponding":false},{"id":1703508,"name":"Yu-Zhong Zhang","orcid":"0000-0002-2017-1005","position":14,"is_corresponding":false},{"id":1703509,"name":"Hai-Nan Su","orcid":"0000-0002-3330-7899","position":15,"is_corresponding":false},{"id":1703498,"name":"Qi Sheng","orcid":"0000-0001-9905-9448","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"In situ visualization of Braun’s lipoprotein on\n            <i>E. coli</i>\n            sacculi","abstract":"<jats:p>\n            Braun’s lipoprotein (Lpp) plays a major role in stabilizing the integrity of the cell envelope in\n            <jats:italic>Escherichia coli</jats:italic>\n            , as it provides a covalent cross-link between the outer membrane and the peptidoglycan layer. An important challenge in elucidating the physiological role of Lpp lies in attaining a detailed understanding of its distribution on the peptidoglycan layer. Here, using atomic force microscopy, we visualized Lpp directly on peptidoglycan sacculi. Lpp is homogeneously distributed over the outer surface of the sacculus at a high density. However, it is absent at the constriction site during cell division, revealing its role in the cell division process with Pal, another cell envelope–associated protein. Collectively, we have established a framework to elucidate the distribution of Lpp and other peptidoglycan-bound proteins via a direct imaging modality.\n          </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36662863","pmcid":"PMC9858504","openalex_id":null,"authors":[],"funders":[{"funder_name":"Wellcome Trust","grant_id":"212197/Z/19/Z","title":null},{"funder_name":"Wellcome Trust","grant_id":"212197/Z/18/Z","title":null},{"funder_name":"Wellcome Trust","grant_id":"212197","title":"The bacterial cell wall in life and death"}],"total_grants":3,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.add8659?download=true","host_type":"publisher"},{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.add8659","host_type":"publisher"},{"url":"https://eprints.whiterose.ac.uk/196806/1/sciadv.add8659.pdf","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9858504/pdf/sciadv.add8659.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9858504","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9858504?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1126/sciadv.add8659","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/36662863","host_type":""},{"url":"http://dx.doi.org/10.1126/sciadv.add8659","host_type":""},{"url":"https://eprints.whiterose.ac.uk/id/eprint/196806/","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Cell Membrane","Escherichia coli","Peptidoglycan","Lipoproteins","Bacterial Outer Membrane Proteins","Microscopy, Atomic Force","Molecular Imaging"],"keywords":["Lipoproteins","Cell Membrane","Escherichia coli","Biomedicine and Life Sciences","Peptidoglycan","Microscopy, Atomic Force","Bacterial Outer Membrane Proteins","Molecular Imaging"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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