{"doi":"10.1126/sciadv.add2058","title":"A genetically encoded far-red fluorescent indicator for imaging synaptically released Zn <sup>2+</sup>","abstract":"Synaptic zinc ion (Zn 2+ ) has emerged as a key neuromodulator in the brain. However, the lack of research tools for directly tracking synaptic Zn 2+ in the brain of awake animals hinders our rigorous understanding of the physiological and pathological roles of synaptic Zn 2+ . In this study, we developed a genetically encoded far-red fluorescent indicator for monitoring synaptic Zn 2+ dynamics in the nervous system. Our engineered far-red fluorescent indicator for synaptic Zn 2+ (FRISZ) displayed a substantial Zn 2+ -specific turn-on response and low-micromolar affinity. We genetically anchored FRISZ to the mammalian extracellular membrane via a transmembrane (TM) ⍺ helix and characterized the resultant FRISZ-TM construct at the mammalian cell surface. We used FRISZ-TM to image synaptic Zn 2+ in the auditory cortex in acute brain slices and awake mice in response to electric and sound stimuli, respectively. Thus, this study establishes a technology for studying the roles of synaptic Zn 2+ in the nervous system.","journal":"Science Advances","year":2023,"id":330954,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":485130,"name":"Manoj Kumar","orcid":"0000-0001-5139-1425","position":1,"is_corresponding":false},{"id":1055310,"name":"Jing Zhang","orcid":"0000-0003-1573-8916","position":2,"is_corresponding":false},{"id":762222,"name":"Shengyu Zhao","orcid":"0000-0002-2501-8464","position":3,"is_corresponding":false},{"id":275972,"name":"Mikhail Drobizhev","orcid":"0000-0001-9072-1300","position":4,"is_corresponding":false},{"id":1055311,"name":"Mason McCollum","orcid":"0000-0002-4251-5913","position":5,"is_corresponding":false},{"id":537995,"name":"Charles T. Anderson","orcid":"0000-0003-3353-0182","position":6,"is_corresponding":false},{"id":1055312,"name":"Ying Wang","orcid":"0000-0001-6370-4134","position":7,"is_corresponding":false},{"id":334294,"name":"Antje Pokorny","orcid":"0000-0001-6675-9966","position":8,"is_corresponding":false},{"id":860720,"name":"Xiaodong Tian","orcid":"0000-0001-9590-9943","position":9,"is_corresponding":false},{"id":860721,"name":"Yiyu Zhang","orcid":"0000-0001-8636-2827","position":10,"is_corresponding":false},{"id":307830,"name":"Thanos Tzounopoulos","orcid":"0000-0003-4583-145X","position":11,"is_corresponding":false},{"id":336191,"name":"Hui‐wang Ai","orcid":"0000-0002-5664-3270","position":12,"is_corresponding":false},{"id":734652,"name":"Tianchen Wu","orcid":"0000-0003-2129-6181","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-19T01:09:14.691473Z","pmid":"36857451","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}