{"doi":"10.1126/sciadv.abq5049","title":"MUC5B mobilizes and MUC5AC spatially aligns mucociliary transport on human airway epithelium","abstract":"<jats:p>Secreted mucus is a frontline defense against respiratory infection, enabling the capture and swift removal of infectious or irritating agents from the lungs. Airway mucus is composed of two mucins: mucin 5B (MUC5B) and 5AC (MUC5AC). Together, they form a hydrogel that can be actively transported by cilia along the airway surface. In chronic respiratory diseases, abnormal expression of these mucins is directly implicated in dysfunctional mucus clearance. Yet, the role of each mucin in supporting normal mucus transport remains unclear. Here, we generate human airway epithelial tissue cultures deficient in either MUC5B or MUC5AC to understand their individual contributions to mucus transport. We find that MUC5B and MUC5AC deficiency results in impaired and discoordinated mucociliary transport, respectively, demonstrating the importance of each mucin to airway clearance.</jats:p>","journal":"Science Advances","year":2022,"id":593972,"datarank":0.611630616585858,"base_score":4.07753744390572,"endowment":4.07753744390572,"self_citation_contribution":0.611630616585858,"citation_network_contribution":0.0,"self_endowment_contribution":0.611630616585858,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":58,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":487116,"name":"Ethan Iverson","orcid":"0000-0001-5815-8079","position":1,"is_corresponding":false},{"id":332708,"name":"Logan Kaler","orcid":"0000-0002-6502-5773","position":2,"is_corresponding":false},{"id":1029207,"name":"Allison Boboltz","orcid":"0000-0002-4203-5928","position":3,"is_corresponding":false},{"id":487118,"name":"Margaret A. Scull","orcid":"0000-0002-6860-2468","position":4,"is_corresponding":false},{"id":332709,"name":"Gregg A. Duncan","orcid":"0000-0002-6811-1327","position":5,"is_corresponding":false},{"id":487117,"name":"Daniel Song","orcid":"0000-0003-2916-6680","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"MUC5B mobilizes and MUC5AC spatially aligns mucociliary transport on human airway epithelium","abstract":"<jats:p>Secreted mucus is a frontline defense against respiratory infection, enabling the capture and swift removal of infectious or irritating agents from the lungs. Airway mucus is composed of two mucins: mucin 5B (MUC5B) and 5AC (MUC5AC). Together, they form a hydrogel that can be actively transported by cilia along the airway surface. In chronic respiratory diseases, abnormal expression of these mucins is directly implicated in dysfunctional mucus clearance. Yet, the role of each mucin in supporting normal mucus transport remains unclear. Here, we generate human airway epithelial tissue cultures deficient in either MUC5B or MUC5AC to understand their individual contributions to mucus transport. We find that MUC5B and MUC5AC deficiency results in impaired and discoordinated mucociliary transport, respectively, demonstrating the importance of each mucin to airway clearance.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36427316","pmcid":"PMC9699686","openalex_id":null,"authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI142050","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"T32 AI125186","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01 HL151840","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01 HL160540","title":null}],"total_grants":4,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by-nc","oa_locations":[{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.abq5049?download=true","host_type":"publisher"},{"url":"https://www.science.org/doi/pdf/10.1126/sciadv.abq5049","host_type":"publisher"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9699686","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9699686","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9699686?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":[],"mesh_terms":["Epithelium","Cilia","Humans","Respiratory Tract Infections","Mucociliary Clearance","Mucin-5B","Mucin 5AC"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T13:02:49.803779Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}