{"doi":"10.1126/sciadv.abo2506","title":"Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer","abstract":"Over the past two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution, which is by a factor of 2 to 3 worse. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging, which achieves an axial resolution down to nanometers. It exploits the distance-dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy ( d STORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of subcellular structures. Moreover, we used spectral demixing for implementing dual-color MIET- d STORM that allows us to image and colocalize, in three dimensions, two different cellular structures simultaneously.","journal":"Science Advances","year":2022,"id":247226,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9531,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":884665,"name":"Marvin Jungblut","orcid":"0009-0003-2950-6961","position":1,"is_corresponding":false},{"id":642054,"name":"Dominic A. Helmerich","orcid":"0009-0001-1889-0406","position":2,"is_corresponding":false},{"id":884666,"name":"Roman Tsukanov","orcid":"0000-0002-3967-1755","position":3,"is_corresponding":false},{"id":885267,"name":"Anna Chizhik","orcid":null,"position":4,"is_corresponding":false},{"id":884667,"name":"Alexey I. Chizhik","orcid":"0000-0003-0454-5924","position":5,"is_corresponding":false},{"id":280793,"name":"Martin J. Schnermann","orcid":"0000-0002-0503-0116","position":6,"is_corresponding":false},{"id":44852,"name":"Markus Sauer","orcid":"0000-0002-1692-3219","position":7,"is_corresponding":false},{"id":884668,"name":"Oleksii Nevskyi","orcid":"0000-0003-2893-481X","position":8,"is_corresponding":false},{"id":884669,"name":"Jörg Enderlein","orcid":"0000-0001-5091-7157","position":9,"is_corresponding":false},{"id":884664,"name":"Jan Christoph Thiele","orcid":"0000-0002-6638-6298","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-19T00:23:53.369530Z","pmid":"35675401","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}