{"doi":"10.1126/sciadv.abn1731","title":"The nonconducting W434F mutant adopts upon membrane depolarization an inactivated-like state that differs from wild-type Shaker-IR potassium channels","abstract":"Voltage-gated K + (Kv) channels mediate the flow of K + across the cell membrane by regulating the conductive state of their activation gate (AG). Several Kv channels display slow C-type inactivation, a process whereby their selectivity filter (SF) becomes less or nonconductive. It has been proposed that, in the fast inactivation-removed Shaker-IR channel, the W434F mutation epitomizes the C-type inactivated state because it functionally accelerates this process. By introducing another pore mutation that prevents AG closure, P475D, we found a way to record ionic currents of the Shaker-IR-W434F-P475D mutant at hyperpolarized membrane potentials as the W434F-mutant SF recovers from its inactivated state. This W434F conductive state lost its high K + over Na + selectivity, and even NMDG + can permeate, features not observed in a wild-type SF. This indicates that, at least during recovery from inactivation, the W434F-mutant SF transitions to a widened and noncationic specific conformation.","journal":"Science Advances","year":2022,"id":261315,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9499,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":916164,"name":"Evelyn Martínez-Morales","orcid":null,"position":1,"is_corresponding":false},{"id":915639,"name":"Dieter V. Van de Sande","orcid":"0000-0003-2377-9240","position":2,"is_corresponding":false},{"id":453616,"name":"Dirk J. Snyders","orcid":"0000-0001-7142-6672","position":3,"is_corresponding":false},{"id":579604,"name":"D. Marien Cortés","orcid":"0000-0001-9733-0679","position":4,"is_corresponding":false},{"id":433744,"name":"Luis G. Cuello","orcid":"0000-0001-5192-7568","position":5,"is_corresponding":false},{"id":453617,"name":"Alain J. Labro","orcid":"0000-0002-0871-7511","position":6,"is_corresponding":false},{"id":453614,"name":"Laura Coonen","orcid":"0000-0002-9047-7288","position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-19T00:26:12.158801Z","pmid":"36112676","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}