{"doi":"10.1126/sciadv.abj8360","title":"The glucocorticoid receptor associates with the cohesin loader NIPBL to promote long-range gene regulation","abstract":"The cohesin complex is central to chromatin looping, but mechanisms by which these long-range chromatin interactions are formed and persist remain unclear. We demonstrate that interactions between a transcription factor (TF) and the cohesin loader NIPBL regulate enhancer-dependent gene activity. Using mass spectrometry, genome mapping, and single-molecule tracking methods, we demonstrate that the glucocorticoid (GC) receptor (GR) interacts with NIPBL and the cohesin complex at the chromatin level, promoting loop extrusion and long-range gene regulation. Real-time single-molecule experiments show that loss of cohesin markedly diminishes the concentration of TF molecules at specific nuclear confinement sites, increasing TF local concentration and promoting gene regulation. Last, patient-derived acute myeloid leukemia cells harboring cohesin mutations exhibit a reduced response to GCs, suggesting that the GR-NIPBL-cohesin interaction is defective in these patients, resulting in poor response to GC treatment.","journal":"Science Advances","year":2022,"id":239221,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":50,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9534,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":628075,"name":"Grégory Fettweis","orcid":"0000-0002-6623-2264","position":1,"is_corresponding":false},{"id":651189,"name":"Sohyoung Kim","orcid":"0000-0002-6140-7918","position":2,"is_corresponding":false},{"id":628072,"name":"David A. Garcia","orcid":"0000-0001-5866-6217","position":3,"is_corresponding":false},{"id":864561,"name":"Saori Fujiwara","orcid":"0000-0002-3531-577X","position":4,"is_corresponding":false},{"id":628073,"name":"Thomas A. Johnson","orcid":"0000-0002-9135-3252","position":5,"is_corresponding":false},{"id":864562,"name":"Theophilus T. Tettey","orcid":"0000-0003-0190-4485","position":6,"is_corresponding":false},{"id":89254,"name":"Laurent Ozbun","orcid":"0000-0002-0862-3929","position":7,"is_corresponding":false},{"id":404288,"name":"Gianluca Pegoraro","orcid":"0000-0003-2843-9464","position":8,"is_corresponding":false},{"id":864563,"name":"Michele Puglia","orcid":"0000-0002-5139-1860","position":9,"is_corresponding":false},{"id":864564,"name":"Blagoy Blagoev","orcid":"0000-0002-3596-0066","position":10,"is_corresponding":false},{"id":246608,"name":"Arpita Upadhyaya","orcid":"0000-0003-1496-919X","position":11,"is_corresponding":false},{"id":579588,"name":"Diana A. Stavreva","orcid":"0000-0002-7904-6452","position":12,"is_corresponding":false},{"id":579589,"name":"Gordon L. Hager","orcid":"0000-0002-9300-5331","position":13,"is_corresponding":false},{"id":628076,"name":"Lorenzo Rinaldi","orcid":"0000-0003-2977-4205","position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":null,"created_at":"2026-07-19T00:22:36.865157Z","pmid":"35353576","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}