{"doi":"10.1126/sciadv.abh0319","title":"Inhalable Nanobody (PiN-21) prevents and treats SARS-CoV-2 infections in Syrian hamsters at ultra-low doses","abstract":"Globally, there is an urgency to develop effective, low-cost therapeutic interventions for coronavirus disease 2019 (COVID-19). We previously generated the stable and ultrapotent homotrimeric Pittsburgh inhalable Nanobody 21 (PiN-21). Using Syrian hamsters that model moderate to severe COVID-19 disease, we demonstrate the high efficacy of PiN-21 to prevent and treat SARS-CoV-2 infection. Intranasal delivery of PiN-21 at 0.6 mg/kg protects infected animals from weight loss and substantially reduces viral burdens in both lower and upper airways compared to control. Aerosol delivery of PiN-21 facilitates deposition throughout the respiratory tract and dose minimization to 0.2 mg/kg. Inhalation treatment quickly reverses animals' weight loss after infection, decreases lung viral titers by 6 logs leading to drastically mitigated lung pathology, and prevents viral pneumonia. Combined with the marked stability and low production cost, this innovative therapy may provide a convenient and cost-effective option to mitigate the ongoing pandemic.","journal":"Science Advances","year":2021,"id":147183,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":171,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9481,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":228171,"name":"Yufei Xiang","orcid":"0000-0002-6732-5649","position":1,"is_corresponding":false},{"id":256403,"name":"Natasha L. Tilston‐Lunel","orcid":"0000-0002-5290-9350","position":2,"is_corresponding":false},{"id":257342,"name":"Linda J. Rennick","orcid":null,"position":3,"is_corresponding":false},{"id":228175,"name":"Zhe Sang","orcid":"0000-0002-1516-2796","position":4,"is_corresponding":false},{"id":210802,"name":"William B. Klimstra","orcid":null,"position":5,"is_corresponding":false},{"id":218442,"name":"Douglas S. Reed","orcid":"0000-0003-0076-9023","position":6,"is_corresponding":false},{"id":559047,"name":"Nicholas A. Crossland","orcid":"0000-0003-3873-9188","position":7,"is_corresponding":false},{"id":228178,"name":"Yi Shi","orcid":"0000-0002-2761-8324","position":8,"is_corresponding":false},{"id":218415,"name":"W. Paul Duprex","orcid":"0000-0003-1716-6376","position":9,"is_corresponding":false},{"id":228172,"name":"Sham Nambulli","orcid":"0000-0002-9781-9703","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-18T23:42:30.206628Z","pmid":"34039613","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}