{"doi":"10.1126/sciadv.abf7613","title":"Engineered yeast tolerance enables efficient production from toxified lignocellulosic feedstocks","abstract":"that engineered aldehyde reduction and elevated extracellular potassium and pH are sufficient to enable near-parity production between inhibitor-laden and inhibitor-free feedstocks. By specifically targeting the universal hydrolysate inhibitors, a single strain is enhanced to tolerate a broad diversity of highly toxified genuine feedstocks and consistently achieve industrial-scale titers (cellulosic ethanol of >100 grams per liter when toxified). Furthermore, a functionally orthogonal, lightweight design enables seamless transferability to existing metabolically engineered chassis strains: We endow full, multifeedstock tolerance on a xylose-consuming strain and one producing the biodegradable plastics precursor lactic acid. The demonstration of \"drop-in\" hydrolysate competence enables the potential of cost-effective, at-scale biomass utilization for cellulosic fuel and nonfuel products alike.","journal":"Science Advances","year":2021,"id":157457,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":50,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9421,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":664500,"name":"Burcu Turanlı-Yıldız","orcid":"0000-0002-3020-9177","position":1,"is_corresponding":false},{"id":664501,"name":"Dany Liu","orcid":"0000-0002-0859-6879","position":2,"is_corresponding":false},{"id":664502,"name":"Michael G. Resch","orcid":"0000-0002-8071-5764","position":3,"is_corresponding":false},{"id":25407,"name":"Gerald R. Fink","orcid":"0000-0003-3704-2899","position":4,"is_corresponding":false},{"id":307074,"name":"Gregory Stephanopoulos","orcid":"0000-0001-6909-4568","position":5,"is_corresponding":false},{"id":664499,"name":"Felix H. Lam","orcid":"0000-0001-6585-3360","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-18T23:44:21.786014Z","pmid":"34172441","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}