{"doi":"10.1126/sciadv.abd4420","title":"Structure of TFIIK for phosphorylation of CTD of RNA polymerase II","abstract":"During transcription initiation, the general transcription factor TFIIH marks RNA polymerase II by phosphorylating Ser5 of the carboxyl-terminal domain (CTD) of Rpb1, which is followed by extensive modifications coupled to transcription elongation, mRNA processing, and histone dynamics. We have determined a 3.5-Å resolution cryo-electron microscopy (cryo-EM) structure of the TFIIH kinase module (TFIIK in yeast), which is composed of Kin28, Ccl1, and Tfb3, yeast homologs of CDK7, cyclin H, and MAT1, respectively. The carboxyl-terminal region of Tfb3 was lying at the edge of catalytic cleft of Kin28, where a conserved Tfb3 helix served to stabilize the activation loop in its active conformation. By combining the structure of TFIIK with the previous cryo-EM structure of the preinitiation complex, we extend the previously proposed model of the CTD path to the active site of TFIIK.","journal":"Science Advances","year":2021,"id":181090,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9445,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":731316,"name":"Tao Li","orcid":"0000-0003-3672-6906","position":1,"is_corresponding":false},{"id":590299,"name":"Hee Jong Kim","orcid":"0000-0002-0823-5301","position":2,"is_corresponding":false},{"id":681400,"name":"Jose J. Gorbea Colón","orcid":"0000-0002-6327-5672","position":3,"is_corresponding":false},{"id":731317,"name":"Mitchell I. Parker","orcid":"0000-0003-1223-3904","position":4,"is_corresponding":false},{"id":56679,"name":"Roland L. Dunbrack","orcid":"0000-0001-7674-6667","position":5,"is_corresponding":false},{"id":74960,"name":"Benjamin A. Garcia","orcid":"0000-0003-3596-4750","position":6,"is_corresponding":false},{"id":636489,"name":"Kuang‐Lei Tsai","orcid":"0000-0002-5428-4219","position":7,"is_corresponding":false},{"id":291118,"name":"Kenji Murakami","orcid":"0000-0002-2017-5575","position":8,"is_corresponding":false},{"id":291115,"name":"Trevor van Eeuwen","orcid":"0000-0003-0549-1702","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-18T23:48:01.257885Z","pmid":"33827808","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}