{"doi":"10.1126/sciadv.abd2408","title":"Assembly of the algal CO <sub>2</sub> -fixing organelle, the pyrenoid, is guided by a Rubisco-binding motif","abstract":", we found that pyrenoid proteins share a sequence motif. We show that the motif is necessary and sufficient to target proteins to the pyrenoid and that the motif binds to Rubisco, suggesting a mechanism for targeting. The presence of the Rubisco-binding motif on proteins that localize to the tubules and on proteins that localize to the matrix-starch sheath interface suggests that the motif holds the pyrenoid's three subcompartments together. Our findings advance our understanding of pyrenoid biogenesis and illustrate how a single protein motif can underlie the architecture of a complex multilayered phase-separated organelle.","journal":"Science Advances","year":2020,"id":54796,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":100,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":283628,"name":"Alan K. Itakura","orcid":null,"position":1,"is_corresponding":false},{"id":259853,"name":"Weronika Patena","orcid":"0000-0002-8388-1303","position":2,"is_corresponding":false},{"id":282147,"name":"Lianyong Wang","orcid":"0000-0003-0906-8346","position":3,"is_corresponding":false},{"id":259845,"name":"Shan He","orcid":"0000-0003-2958-9659","position":4,"is_corresponding":false},{"id":282148,"name":"Tom Z. Emrich‐Mills","orcid":"0000-0002-4440-626X","position":5,"is_corresponding":false},{"id":283629,"name":"Chun Sing Lau","orcid":null,"position":6,"is_corresponding":false},{"id":282149,"name":"Gary F. Yates","orcid":"0000-0002-1301-2452","position":7,"is_corresponding":false},{"id":282150,"name":"Luke C. M. Mackinder","orcid":"0000-0003-1440-3233","position":8,"is_corresponding":false},{"id":253773,"name":"Martin C. Jonikas","orcid":"0000-0002-9519-6055","position":9,"is_corresponding":false},{"id":259848,"name":"Moritz T. Meyer","orcid":"0000-0001-8516-2591","position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-18T21:04:14.743014Z","pmid":"33177094","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}