{"doi":"10.1126/sciadv.abb8219","title":"Structural insight into the <i>Staphylococcus aureus</i> ATP-driven exporter of virulent peptide toxins","abstract":"is a major human pathogen that has acquired alarming broad-spectrum antibiotic resistance. One group of secreted toxins with key roles during infection is the phenol-soluble modulins (PSMs). PSMs are amphipathic, membrane-destructive cytolytic peptides that are exported to the host-cell environment by a designated adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter, the PSM transporter (PmtABCD). Here, we demonstrate that the minimal Pmt unit necessary for PSM export is PmtCD and provide its first atomic characterization by single-particle cryo-EM and x-ray crystallography. We have captured the transporter in the ATP-bound state at near atomic resolution, revealing a type II ABC exporter fold, with an additional cytosolic domain. Comparison to a lower-resolution nucleotide-free map displaying an \"open\" conformation and putative hydrophobic inner chamber of a size able to accommodate the binding of two PSM peptides provides mechanistic insight and sets the foundation for therapeutic design.","journal":"Science Advances","year":2020,"id":68641,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9634,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":363789,"name":"Seth W. Dickey","orcid":"0000-0003-3200-2388","position":1,"is_corresponding":false},{"id":363790,"name":"Jinhong Hu","orcid":"0000-0003-3626-9602","position":2,"is_corresponding":false},{"id":259846,"name":"Hui‐Ting Chou","orcid":"0000-0002-8693-8571","position":3,"is_corresponding":false},{"id":241698,"name":"L.J. Worrall","orcid":"0000-0003-4966-7175","position":4,"is_corresponding":false},{"id":363791,"name":"J. Andrew N. Alexander","orcid":"0000-0002-8759-1389","position":5,"is_corresponding":false},{"id":363792,"name":"Michael Luke Carlson","orcid":"0000-0002-3807-6516","position":6,"is_corresponding":false},{"id":363793,"name":"Michael L. Nosella","orcid":"0000-0003-2408-3050","position":7,"is_corresponding":false},{"id":363794,"name":"Franck Duong","orcid":"0000-0001-7328-6124","position":8,"is_corresponding":false},{"id":259858,"name":"Zhiheng Yu","orcid":"0000-0002-7114-2570","position":9,"is_corresponding":false},{"id":268488,"name":"Michaël Otto","orcid":"0000-0002-2222-4115","position":10,"is_corresponding":false},{"id":241705,"name":"N.C.J. Strynadka","orcid":"0000-0002-4058-9425","position":11,"is_corresponding":false},{"id":363788,"name":"Natalie Zeytuni","orcid":"0000-0002-5708-4095","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-18T21:41:52.279368Z","pmid":"32998902","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}