{"doi":"10.1126/sciadv.aaz0108","title":"Adverse effects of electronic cigarettes on the disease-naive oral microbiome","abstract":"Six percent of Americans, including 3 million high schoolers, use e-cigarettes, which contain potentially toxic substances, volatile organic compounds, and metals. We present the first human study on the effects of e-cigarette exposure in the oral cavity. By interrogating both immunoinflammatory responses and microbial functional dynamics, we discovered pathogen overrepresentation, higher virulence signatures, and a brisk proinflammatory signal in clinically healthy e-cigarette users, equivalent to patients with severe periodontitis. Using RNA sequencing and confocal and electron microscopy to validate these findings, we demonstrate that the carbon-rich glycol/glycerol vehicle is an important catalyst in transforming biofilm architecture within 24 hours of exposure. Last, a machine-learning classifier trained on the metagenomic signatures of e-cigarettes identified as e-cigarette users both those individuals who used e-cigarettes to quit smoking, and those who use both e-cigarettes and cigarettes. The present study questions the safety of e-cigarettes and the harm reduction narrative promoted by advertising campaigns.","journal":"Science Advances","year":2020,"id":53706,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":113,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9528,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":274561,"name":"Shareef M. Dabdoub","orcid":"0000-0002-1374-8347","position":1,"is_corresponding":false},{"id":274562,"name":"Haikady N. Nagaraja","orcid":"0000-0001-8896-0804","position":2,"is_corresponding":false},{"id":274563,"name":"Michelle L. Scott","orcid":"0000-0003-0925-5942","position":3,"is_corresponding":false},{"id":276413,"name":"Surya Pamulapati","orcid":null,"position":4,"is_corresponding":false},{"id":274564,"name":"Micah L. Berman","orcid":"0000-0002-0336-1317","position":5,"is_corresponding":false},{"id":274565,"name":"Peter G. Shields","orcid":"0000-0002-7404-217X","position":6,"is_corresponding":false},{"id":276414,"name":"Mary Ellen Wewers","orcid":null,"position":7,"is_corresponding":false},{"id":274566,"name":"Purnima Kumar","orcid":"0000-0001-5844-1341","position":8,"is_corresponding":false},{"id":274560,"name":"Sukirth M. Ganesan","orcid":"0000-0002-6303-7287","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-07-18T20:45:25.145885Z","pmid":"32518820","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}