{"doi":"10.1126/sciadv.aay9093","title":"A facile technology for the high-throughput sequencing of the paired VH:VL and TCRβ:TCRα repertoires","abstract":"Natively paired sequencing (NPS) of B cell receptors [variable heavy (VH) and light (VL)] and T cell receptors (TCRb and TCRa) is essential for the understanding of adaptive immunity in health and disease. Despite many recent technical advances, determining the VH:VL or TCRb:a repertoire with high accuracy and throughput remains challenging. We discovered that the recently engineered xenopolymerase, RTX, is exceptionally resistant to cell lysate inhibition in single-cell emulsion droplets. We capitalized on the characteristics of this enzyme to develop a simple, rapid, and inexpensive in-droplet overlap extension reverse transcription polymerase chain reaction method for NPS not requiring microfluidics or other specialized equipment. Using this technique, we obtained high yields (5000 to >20,000 per sample) of paired VH:VL or TCRb:a clonotypes at low cost. As a demonstration, we performed NPS on peripheral blood plasmablasts and T follicular helper cells following seasonal influenza vaccination and discovered high-affinity influenza-specific antibodies and TCRb:a.","journal":"Science Advances","year":2020,"id":75233,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9504,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":392854,"name":"Jonathan R. McDaniel","orcid":"0000-0001-8575-3535","position":1,"is_corresponding":false},{"id":392855,"name":"Christopher Stevens","orcid":"0000-0003-3178-3290","position":2,"is_corresponding":false},{"id":247618,"name":"William N. Voss","orcid":"0000-0002-4403-1939","position":3,"is_corresponding":false},{"id":392856,"name":"Jie Li","orcid":"0000-0002-7170-1897","position":4,"is_corresponding":false},{"id":394099,"name":"R. Durrett","orcid":null,"position":5,"is_corresponding":false},{"id":392857,"name":"Jiwon Lee","orcid":"0000-0001-7835-3621","position":6,"is_corresponding":false},{"id":235707,"name":"Jimmy Gollihar","orcid":"0000-0003-3957-2092","position":7,"is_corresponding":false},{"id":392858,"name":"Yuri Tanno","orcid":"0000-0002-1868-7168","position":8,"is_corresponding":false},{"id":392859,"name":"George Delidakis","orcid":"0000-0002-4330-9476","position":9,"is_corresponding":false},{"id":394100,"name":"Arti Pothukuchy","orcid":null,"position":10,"is_corresponding":false},{"id":394101,"name":"Jared W. Ellefson","orcid":null,"position":11,"is_corresponding":false},{"id":235213,"name":"Jörg J. Goronzy","orcid":"0000-0001-7670-1856","position":12,"is_corresponding":false},{"id":105934,"name":"Jennifer A. Maynard","orcid":"0000-0002-0363-8486","position":13,"is_corresponding":false},{"id":392860,"name":"Andrew D. Ellington","orcid":"0000-0001-6246-5338","position":14,"is_corresponding":false},{"id":105933,"name":"Gregory C. Ippolito","orcid":"0000-0002-7565-7002","position":15,"is_corresponding":false},{"id":106348,"name":"George Georgiou","orcid":"0000-0002-8823-3073","position":16,"is_corresponding":false},{"id":392853,"name":"Hidetaka Tanno","orcid":"0000-0001-6749-0938","position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":null,"created_at":"2026-07-18T21:46:33.313355Z","pmid":"32426460","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}