{"doi":"10.1124/mol.56.1.11","title":"Functional α6-Containing Nicotinic Receptors Are Present in Chick Retina","abstract":null,"journal":"Molecular Pharmacology","year":1999,"id":665990,"datarank":0.7130385286659547,"base_score":4.7535901911063645,"endowment":4.7535901911063645,"self_citation_contribution":0.7130385286659547,"citation_network_contribution":0.0,"self_endowment_contribution":0.7130385286659547,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":115,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1739134,"name":"Wolfgang Hanke","orcid":null,"position":1,"is_corresponding":false},{"id":1739135,"name":"Andreea Bejan","orcid":null,"position":2,"is_corresponding":false},{"id":1739136,"name":"Benedetta Barabino","orcid":null,"position":3,"is_corresponding":false},{"id":1739137,"name":"Renato Longhi","orcid":null,"position":4,"is_corresponding":false},{"id":1739138,"name":"Barbara Balestra","orcid":null,"position":5,"is_corresponding":false},{"id":349649,"name":"Milena Moretti","orcid":null,"position":6,"is_corresponding":false},{"id":349652,"name":"Francesco Clementi","orcid":null,"position":7,"is_corresponding":false},{"id":348547,"name":"Cecilia Gotti","orcid":"0000-0001-5358-8643","position":8,"is_corresponding":false},{"id":1739133,"name":"Silvia Vailati","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Functional α6-Containing Nicotinic Receptors Are Present in Chick Retina","abstract":"Despite the fact that the neuronal chick alpha6 subunit was first cloned several years ago and recently has been shown to form acetylcholine (ACh)-activated channels in heterologous systems, no information is yet available concerning the structure and function of the alpha6-containing nicotinic receptors in neuronal tissues. Using subunit-specific antibodies directed against two different epitopes of the chick alpha6 subunit, we performed immunoprecipitation experiments on immunopurified alpha6-containing receptors radiolabeled with the nicotinic agonist [3H]epibatidine (Epi): almost all of the alpha6 receptors contained the beta4 subunit, 51% the beta3 subunit, 42% the alpha3 subunit, and 7.5% the beta2 subunit. Western blot analyses of the purified receptors confirmed the presence of the alpha3, beta3, beta2, and beta4 subunits, and the absence of the alpha4, alpha5, and alpha7 subunits. The alpha6-containing receptors bind [3H]Epi (Kd = 35 pM) and a number of other nicotinic agonists with very high affinity, the rank order being Epi >> cytisine > nicotine > 1, 1-dimethyl-4-phenylpiperazinium > acetylcholine > carbamylcholine. The alpha6 receptors also have a distinct antagonist pharmacological profile with a rank order of potency of alpha-conotoxin MII > methyllycaconitine > dihydro-beta-erythroydine > MG624 > d-tubocurarine > decamethonium > hexamethonium. When reconstituted in lipid bilayers, the alpha6-containing receptors form functional cationic channels with a main conductance state of 48 pS. These channels are activated by nicotinic agonists in a dose-dependent manner, and blocked by the nicotinic antagonist d-tubocurarine.","is_dataset_classified":null,"base_score":4.7535901911063645,"endowment":4.7535901911063645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10385679","pmcid":null,"openalex_id":"https://openalex.org/W1919231994","authors":[],"funders":[],"total_grants":0,"fwci":5.2171,"citation_percentile":0.96304836,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2014,"count":1},{"year":2015,"count":1},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://syndication.highwire.org/content/doi/10.1124/mol.56.1.11","host_type":"publisher"},{"url":"https://doi.org/10.1124/mol.56.1.11","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10385679","host_type":"repository"}],"fields_of_study":["Nicotinic Acetylcholine Receptors Study","Ion channel regulation and function","Insect and Pesticide Research","Amino Acid Sequence","Animals","Antibodies","Chickens","Lipid Bilayers","Membrane Potentials","Molecular Sequence Data","Receptors, Nicotinic","Retina","Tectum Mesencephali"],"mesh_terms":["Amino Acid Sequence","Animals","Antibodies","Chickens","Tectum Mesencephali","Lipid Bilayers","Membrane Potentials","Molecular Sequence Data","Receptors, Nicotinic","Retina"],"keywords":["Methyllycaconitine","Nicotinic agonist","Epibatidine","Nicotinic Antagonist","Alpha-4 beta-2 nicotinic receptor","Receptor","Ganglion type nicotinic receptor","Acetylcholine receptor","Cytisine","Cys-loop receptors","Hexamethonium","Protein subunit","Biology","Chemistry","Nicotinic acetylcholine receptor","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T12:54:12.548028Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}