{"doi":"10.1117/12.762058","title":"The mechanism of PDT-induced electrical blockade: the measurement of intracellular Ca<sup>2+</sup>concentration changes in cardiac myocytes","abstract":null,"journal":"SPIE Proceedings","year":2008,"id":658412,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1718768,"name":"S. Hosokawa","orcid":null,"position":1,"is_corresponding":false},{"id":1718771,"name":"S. Hakomori","orcid":null,"position":2,"is_corresponding":false},{"id":1718774,"name":"S. Miyoshi","orcid":null,"position":3,"is_corresponding":false},{"id":1718776,"name":"K. Soejima","orcid":null,"position":4,"is_corresponding":false},{"id":159942,"name":"T. Arai","orcid":null,"position":5,"is_corresponding":false},{"id":1718766,"name":"A. Ito","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The mechanism of PDT-induced electrical blockade: the measurement of intracellular Ca<sup>2+</sup>concentration changes in cardiac myocytes","abstract":"We propose the application of early state photodynamic therapy (PDT) to treatment of atrial fibrillation, which is a kind of arrhythmia characterized by irregular rapid beating of heart. We had demonstrated that our PDT can block the propagation of electrical excitation in cardiac myocytes. However, the mechanism of the PDT-induced electrical blockade was not clear. In order to clarify this mechanism, changes in intracellular Ca<sup>2+</sup> concentration during the PDT with Talaporfin sodium (water soluble photosensitizer) were measured by fluorescence Ca<sup>2+</sup> indicator, fluo-4 AM. The PDT led to the rapid increase of intracellular Ca<sup>2+</sup> concentration and the changes in cell shapes. These results indicated that extracellular Ca<sup>2+</sup> flowed into the cells mediated by cell membrane. Moreover, we found bubble generation in the cells after the PDT. In conclusion, the PDT-induced electrical blockade in myocytes can be caused by cell death following the bubble generation, which is accompanied by the increase in intracellular Ca<sup>2+</sup> concentration due to the cell membrane malfunction with the PDT.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":"https://openalex.org/W2092476470","authors":[],"funders":[],"total_grants":0,"fwci":6.7658,"citation_percentile":0.96035242,"influential_citations":0,"citation_trend":[{"year":2015,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://doi.org/10.1117/12.762058","host_type":"journal"}],"fields_of_study":["Analytical Chemistry and Sensors","bioluminescence and chemiluminescence research","Photodynamic Therapy Research Studies"],"mesh_terms":[],"keywords":["Intracellular","Extracellular","Biophysics","Photodynamic therapy","Photosensitizer","Myocyte","Cell membrane","Materials science","Cell","Chemistry","Medicine","Internal medicine","Biology","Biochemistry","Photochemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T04:09:14.145854Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}