{"doi":"10.1117/1.jbo.30.4.046001","title":"Dual-channel pulse-dye densitometry can enable correction of fluorescent targeted and control agent plasma input function differences for quantitative paired-agent molecular imaging: a simulation study","abstract":"Significance: Paired-agent fluorescent molecular imaging approaches involve co-administration of a control (untargeted) imaging agent with a molecularly targeted agent to account for non-specific effects and quantify binding potential (BP)-a parameter proportional to the concentration of the targeted biomolecule. Accurate BP estimation often requires correction for differences in targeted and control agent plasma input functions (PIFs). Aim: We provide a simulation-based evaluation of whether dual-channel pulse dye densitometry (PDD) can be used to measure the PIFs of co-administered targeted and control imaging agents, to enable accurate BP estimation. Approach: Monte-Carlo simulations of light propagation were carried out using the anatomy and optical properties of a finger, as well as experimentally measured PIFs of co-administered anti-epidermal growth factor receptor fluorescent affibody, ABY-029, and IRDye 680LT, a control imaging agent from past mouse experiments. The accuracy of PIF shape estimation from PDD and PIF difference correction was evaluated by assessing BP estimation accuracy in a simulated \"tumor\" tissue. Results: . No statistical difference was found between the estimated BP via deconvolution correction with true PIFs and the estimated BP via the reconstructed PIFs using the proposed PAF-PDD methodology. Conclusions: These results highlight the potential for developing a PDD instrument that can directly measure targeted and control agent PIFs and be used to correct for any PIF differences between agents for more quantitative estimates of BP in paired-agent imaging studies.","journal":"Journal of Biomedical Optics","year":2025,"id":563491,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9552,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1113111,"name":"Yichen Feng","orcid":null,"position":1,"is_corresponding":false},{"id":1112719,"name":"Sanjana Pannem","orcid":"0009-0005-0690-4224","position":2,"is_corresponding":false},{"id":1143592,"name":"Jovan G. Brankov","orcid":"0000-0001-6398-4711","position":3,"is_corresponding":false},{"id":1466559,"name":"Kimberly S. Samkoe","orcid":null,"position":4,"is_corresponding":false},{"id":511976,"name":"Kenneth M. Tichauer","orcid":"0000-0003-1667-3026","position":5,"is_corresponding":false},{"id":1143589,"name":"Cody C. Rounds","orcid":"0009-0003-2586-2731","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T02:56:13.374766Z","pmid":"40161250","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}