{"doi":"10.1117/1.bios.3.1.012902","title":"Real-time processing of high-throughput quantitative phase microscopy data using a Jetson Orin Nano","abstract":"Significance: Quantitative phase microscopy (QPM) is a holographic imaging technique often applied to studying cell morphology. To advance QPM for clinical applications, high-throughput implementations have been developed to allow imaging of thousands of cells at a time. Aim: To meet the needs of processing raw data and creating QPM holographic images, higher throughput processing methods are needed. Here, we report on the use of a system-on-module approach for QPM data processing. Approach: We have developed a real-time processing pipeline that leverages the parallel processing capabilities of the NVIDIA Jetson Orin Nano to implement processing of cell data. We demonstrate this pipeline on a holographic cytometry (HC) system, a high-throughput QPM implementation. The CUDA processing algorithm enables the generation of QPM data from raw interferograms followed by phase unwrapping, cell segmentation, and refocusing. Result: We captured, processed, and analyzed 107,631 red blood cell images. The processing speed reaches 1200 cells/s in the speed test. Benchmarking shows that real-time refocusing maintains a high degree of structural similarity to the traditional refocusing method. Conclusion: The result demonstrates that our pipeline could accelerate the statistical analysis of cell populations. We expect this study to benefit the development of a portable, low-cost HC system.","journal":"Biophotonics discovery.","year":2025,"id":578670,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9534,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1212601,"name":"Robert E. Highland","orcid":"0009-0007-3468-3224","position":1,"is_corresponding":false},{"id":1488947,"name":"Justin Fan","orcid":null,"position":2,"is_corresponding":false},{"id":1488591,"name":"David A. Miller","orcid":"0000-0002-0033-6298","position":3,"is_corresponding":false},{"id":505338,"name":"Adam Wax","orcid":"0000-0002-1827-5112","position":4,"is_corresponding":false},{"id":1377038,"name":"Wan Wang","orcid":"0000-0002-9847-2887","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:58:24.957414Z","pmid":"42028047","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}