{"doi":"10.1111/xen.70009","title":"Preservation of Cardiac Xenografts in a Model of Infant Human Cardiac Transplantation","abstract":"INTRODUCTION: There is no standard protocol for management of organ preservation for orthotopic, life-sustaining cardiac xenotransplantation, particularly for hearts from pediatric sized donors. Standard techniques and solutions successful in human allotransplantation are not viable. We theorized that a solution commonly used in reparative cardiac surgery in human children would suffice by exploiting the advantages inherent to xenotransplantation, namely the ability to reduce organ ischemic times by co-locating the donor and recipient. METHODS: Orthotopic cardiac xenotransplantation was performed from genetically engineered pigs to size matched baboons. A dose of modified Del Nido cardioplegia initiated donor heart arrest and was followed by a second dose mixed with recipient blood prior to implant. Hemodynamics and cardiac function were tracked with a combination of invasive and non-invasive measures. RESULTS: Mean ischemic time and cardiopulmonary bypass times were 54.1 ± 14.6 and 84.1 ± 14 min respectively. The ejection fraction following chest closure was preserved at >50% for all animals. This finding persisted at 48hours. Mean inotropic score at 24 h post-implant was 9.7 ± 3. CONCLUSION: Del Nido cardioplegia solution when combined with short graft ischemic times demonstrates promising outcomes to avoid primary graft dysfunction for cardiac xenografts in a small animal model of life-sustaining orthotopic cardiac xenotransplantation. Ex vivo perfusion systems may be unnecessary for successful clinical implementation of this evolving technology.","journal":"Xenotransplantation","year":2025,"id":530179,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9464,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":418554,"name":"Joe H. Simmons","orcid":"0000-0003-1815-6831","position":1,"is_corresponding":false},{"id":1386687,"name":"C. Vo","orcid":"0000-0002-6286-4635","position":2,"is_corresponding":false},{"id":1387131,"name":"Kanwarpal Bakshi","orcid":null,"position":3,"is_corresponding":false},{"id":1387132,"name":"Julie Juliani","orcid":null,"position":4,"is_corresponding":false},{"id":1386688,"name":"Julie Fenske","orcid":"0000-0002-7520-5110","position":5,"is_corresponding":false},{"id":379341,"name":"Carolyn L. Hodo","orcid":"0000-0003-4113-7081","position":6,"is_corresponding":false},{"id":797873,"name":"David C. Cleveland","orcid":"0000-0002-7954-3512","position":7,"is_corresponding":false},{"id":54934,"name":"John D. Cleveland","orcid":null,"position":8,"is_corresponding":false},{"id":1311039,"name":"Chace Mitchell","orcid":"0000-0002-1578-726X","position":0,"is_corresponding":true}],"reference_count":10,"raw_metadata":null,"created_at":"2026-07-19T02:51:05.836974Z","pmid":"39786348","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}