{"doi":"10.1111/trf.18286","title":"Optimization of biotinylation protocol for next generation studies of red blood cell survival after transfusion","abstract":"BACKGROUND: Red blood cell (RBC) biotinylation has increasingly become the preferred method for quantifying RBC post-transfusion recovery (PTR). This study evaluated the feasibility of transfusing autologous RBCs labeled with biotin (BioRBCs) 48 h prior to transfusion. The rationale was to facilitate the distribution of BioRBC products from manufacturing sites to remote clinical sites. STUDY DESIGN: Leukocyte-reduced RBC units from 12 healthy individuals were stored at 1-6°C for 42 days and biotinylated with two biotin densities (3 and 15 μg/mL) 48 and 4 h prior to transfusion. Each individual was transfused with 10 mL of each BioRBC preparation, after which BioRBC 20 h PTR and long-term survival (30 and 90 days) were determined by flow cytometric analyses. Additional quality measurements included phosphatidylserine (PS) exposure on BioRBCs and in vitro metrics of hemolysis. RESULTS: RBC transfusion 48 h after biotinylation was not associated with altered BioRBC 20 h PTR or 30 and 90 day survival as compared with RBC transfusion 4 h after biotinylation (e.g., PTR 20 h: 85.7% ± 8.4% vs. 87.5% ± 7.3%; 48 and 4 h, respectively, p > .05). Similarly, no significant differences between the BioRBC groups were observed in BioRBC PS exposure at all time points. BioRBC long-term survival, but not 20 h PTR, was negatively associated with donor hemoglobin (Pearson r = -0.71, p = .001) and osmotic hemolysis (r = -0.783, p = .003). None of the participants developed antibodies against BioRBCs during the trial. CONCLUSIONS: RBC biotinylation 48 h prior to transfusion does not compromise the quality and safety of BioRBC products. BioRBC long-term survival can be used to identify donor characteristics that influence RBC lifespan in the circulation.","journal":"Transfusion","year":2025,"id":541242,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9615,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":806802,"name":"Gemlyn George","orcid":"0000-0002-4518-0629","position":1,"is_corresponding":false},{"id":1429991,"name":"Joseph Sekiya","orcid":null,"position":2,"is_corresponding":false},{"id":621501,"name":"Kathleen Kelly","orcid":"0000-0002-1102-6279","position":3,"is_corresponding":false},{"id":1429992,"name":"Crystal Stanley","orcid":null,"position":4,"is_corresponding":false},{"id":798610,"name":"Deborah Lee","orcid":null,"position":5,"is_corresponding":false},{"id":357724,"name":"Jeffrey M. Finlon","orcid":null,"position":6,"is_corresponding":false},{"id":1429620,"name":"M.A. Rahman","orcid":"0000-0002-0468-2518","position":7,"is_corresponding":false},{"id":1429993,"name":"Timothy Gehris","orcid":null,"position":8,"is_corresponding":false},{"id":1429621,"name":"Dylan Bowen","orcid":"0009-0007-5905-5531","position":9,"is_corresponding":false},{"id":1137415,"name":"Michelle Barton","orcid":"0000-0001-7707-8154","position":10,"is_corresponding":false},{"id":757454,"name":"Susanne Marschner","orcid":"0000-0002-7862-6201","position":11,"is_corresponding":false},{"id":758892,"name":"Mary Berg","orcid":"0000-0002-4961-245X","position":12,"is_corresponding":false},{"id":284370,"name":"Philip J. Norris","orcid":"0000-0003-0526-2088","position":13,"is_corresponding":false},{"id":1429994,"name":"for the Recipient Epidemiology and Donor Evaluation Study‐IV‐Pediatric (REDS‐IV‐P)","orcid":null,"position":14,"is_corresponding":false},{"id":257584,"name":"Tamir Kanias","orcid":"0000-0001-6558-0913","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-19T02:52:47.161928Z","pmid":"40390385","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}