{"doi":"10.1111/trf.17168","title":"Absence of pathogenic viruses in <scp>COVID</scp>‐19 convalescent plasma","abstract":"BACKGROUND: It is important to maintain the safety of blood products by avoiding the transfusion of units with known and novel viral pathogens. It is unknown whether COVID-19 convalescent plasma (CCP) may contain pathogenic viruses (either newly acquired or reactivated) that are not routinely screened for by blood centers. METHODS: The DNA virome was characterized in potential CCP donors (n = 30) using viral genome specific PCR primers to identify DNA plasma virome members of the Herpesviridae [Epstein Barr Virus (EBV), cytomegalovirus (CMV), human herpesvirus 6A/B, human herpesvirus 7] and Anelloviridae [Torque teno viruses (TTV), Torque teno mini viruses (TTMV), and Torque teno midi viruses (TTMDV)] families. In addition, the RNA plasma virome was characterized using unbiased metagenomic sequencing. Sequencing was done on a HiSeq2500 using high output mode with a read length of 2X100 bp. The sequencing reads were taxonomically classified using Kraken2. CMV and EBV seroprevalence were evaluated using a chemiluminescent immunoassay. RESULTS: TTV and TTMDV were detected in 12 (40%) and 4 (13%) of the 30 study participants, respectively; TTMDV was always associated with infection with TTV. We did not observe TTMV DNAemia. Despite CMV and EBV seroprevalences of 33.3% and 93.3%, respectively, we did not detect Herpesviridae DNA among the study participants. Metagenomic sequencing did not reveal any human RNA viruses in CCP, including no evidence of circulating SARS-CoV-2. DISCUSSION: There was no evidence of pathogenic viruses, whether newly acquired or reactivated, in CCP despite the presence of non-pathogenic Anelloviridae. These results confirm the growing safety data supporting CCP.","journal":"Transfusion","year":2022,"id":286842,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9477,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":227504,"name":"Sarah E. Benner","orcid":"0000-0002-8336-2976","position":1,"is_corresponding":false},{"id":227514,"name":"Evan M. Bloch","orcid":"0000-0001-8181-9517","position":2,"is_corresponding":false},{"id":229686,"name":"Ruchee Shrestha","orcid":null,"position":3,"is_corresponding":false},{"id":821960,"name":"Olivia Ajayi","orcid":null,"position":4,"is_corresponding":false},{"id":353472,"name":"Xianming Zhu","orcid":"0000-0002-3317-7449","position":5,"is_corresponding":false},{"id":108424,"name":"Patrizio Caturegli","orcid":"0000-0001-6768-6308","position":6,"is_corresponding":false},{"id":227512,"name":"Shmuel Shoham","orcid":"0000-0001-7276-7786","position":7,"is_corresponding":false},{"id":227511,"name":"David Sullivan","orcid":"0000-0003-0319-0578","position":8,"is_corresponding":false},{"id":311291,"name":"Kelly A. Gebo","orcid":"0000-0003-4010-398X","position":9,"is_corresponding":false},{"id":227510,"name":"Thomas C. Quinn","orcid":"0000-0002-0404-1315","position":10,"is_corresponding":false},{"id":107237,"name":"Arturo Casadevall","orcid":"0000-0002-9402-9167","position":11,"is_corresponding":false},{"id":301017,"name":"Daniel F. Hanley","orcid":"0000-0002-9536-4258","position":12,"is_corresponding":false},{"id":227501,"name":"Andrew Pekosz","orcid":"0000-0003-3248-1761","position":13,"is_corresponding":false},{"id":227513,"name":"Andrew D. Redd","orcid":"0000-0003-4651-1423","position":14,"is_corresponding":false},{"id":434568,"name":"Ashwin Balagopal","orcid":"0000-0002-6143-3104","position":15,"is_corresponding":false},{"id":227515,"name":"Aaron A.R. Tobian","orcid":"0000-0002-0517-3766","position":16,"is_corresponding":false},{"id":452557,"name":"Abraham J. Kandathil","orcid":"0000-0002-9038-134X","position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":null,"created_at":"2026-07-19T00:29:56.781756Z","pmid":"36268708","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}