{"doi":"10.1111/tra.12724","title":"Components of the G <sub>s</sub> signaling cascade exhibit distinct changes in mobility and membrane domain localization upon β <sub>2</sub> ‐adrenergic receptor activation","abstract":"Abstract The G protein signaling cascade is a key player in cell signaling. Cascade activation leads to a redistribution of its members in various cellular compartments. These changes are likely related to the “second wave” of signaling from endosomes. Here, we set out to determine whether G s signaling cascade members expressed at very low levels exhibit altered mobility and localize in clathrin‐coated structures (CCSs) or caveolae upon activation by β 2 ‐adrenergic receptors (β 2 AR). Activated β 2 AR showed decreased mobility and sustained accumulation in CCSs but not in caveolae. Arrestin 3 translocated to the plasma membrane after β 2 AR activation and showed very low mobility and pronounced accumulation in CCSs. In contrast, Gα s and Gγ 2 exhibited a modest reduction in mobility but no detectable accumulation in or exclusion from CCSs or caveolae. The effector adenylyl cyclase 5 (AC5) showed a slight mobility increase upon β 2 AR stimulation, no redistribution to CCSs, and weak activation‐independent accumulation in caveolae. Our findings show an overall decrease in the mobility of most activated G s signaling cascade members and confirm that β 2 AR and arrestin 3 accumulate in CCSs, while Gα s , Gγ 2 and AC5 can transiently enter CCSs and caveolae but do not accumulate in and are not excluded from these domains.","journal":"Traffic","year":2020,"id":74378,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9644,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":268406,"name":"Wonjo Jang","orcid":"0000-0002-1928-8978","position":1,"is_corresponding":false},{"id":389003,"name":"Ekaterina Sviridova","orcid":"0009-0009-9956-2030","position":2,"is_corresponding":false},{"id":268407,"name":"Nevin A. Lambert","orcid":"0000-0001-7550-0921","position":3,"is_corresponding":false},{"id":389002,"name":"Alexey Bondar","orcid":"0000-0002-1980-2930","position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":null,"created_at":"2026-07-18T21:45:52.171053Z","pmid":"32096320","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}